Table 6.

miRNAs modulation in breast cancer tissues versus normal tissues in the 1,359 patients with breast cancer from the METABRIC study and in subtypes of breast cancer tissues versus normal tissues derived from the highly characterized subgroup of 81 participants

P value for all subtyped togetherP value for matched samples by subtype
Expression in Caldas dataset (METABRIC)Direction of changeN vs. T (81 vs. 1,278)Matched N vs. T (81)Normal-like (15)Her2 (5)Basal-like (15)Lum A (27)Lum B (19)KM P value
Downregulated miRNAs
 hsa-miR125a-5pExpressedUpregulated in T<0.001<0.0010.8140.1100.724<0.001<0.0010.791
 hsa-miR141ExpressedUpregulated in T<0.001<0.0010.1980.0560.445<0.001<0.0010.0637
 hsa-miR582-5pExpressedUpregulated in T<0.001<0.0010.4210.5030.4830.0040.1300.144
 hsa-miR138Not Expressed
 hsa-miR199a-5pExpressedNo difference0.1090.3040.7430.0790.6150.0940.0180.0043
 hsa-miR181c*ExpressedNo difference0.0630.4890.4700.0080.3530.0180.9870.00857
 hsa-miR28-3pNot Expressed
 hsa-miR224ExpressedDownregulated in T<0.001<0.001<0.0010.5980.511<0.001<0.0010.202
 hsa-miR145-3pExpressedDownregulated in T<0.001<0.001<0.0010.0610.0120.002<0.0010.00121
 hsa-miR145-5pExpressedDownregulated in T<0.001<0.0010.0050.007<0.001<0.001<0.0010.112
 hsa-miR223ExpressedNo difference0.1690.3640.3720.4270.1010.8700.4980.0086
 hsa-miR539ExpressedDownregulated in T<0.0010.0810.2630.5700.9740.7470.0080.0113
 hsa-miR99bExpressedDownregulated in T0.0030.4390.3150.1140.6730.3920.1600.0197
 hsa-miR199b-5pExpressedDownregulated in T<0.0010.0030.0080.1840.1250.4760.012<0.001
 hsa-miR920Not expressed
Upregulated miRNAs
 hsa-miR892bNot expressed
 hsa-miR1288Not expressed
 hsa-miR520a-3pNot expressed
 hsa-miR542-5pExpressedNo difference0.1220.2490.5650.2560.0600.0950.1030.0188
 hsa-miR122*Not expressed

NOTE: For each miRNA, it is indicated whether it is expressed in the METABRIC dataset (column 2); for the expressed miRNAs, it is also indicated whether they are over- or underexpressed in the tumor tissue relative to normal samples (column 3), and corresponding P values for all samples together (column 4), only in matched tumor and normal samples from the same patient across all subtypes (column 5), and for each subtype alone (columns 6–10). The number of patients in each comparison is indicated in parentheses in the column heads. The last column contains P values for Kaplan–Meier analysis, comparing the difference in survival between the third of patients with the highest expression of the miRNA to the third of patients with lowest expression.

Abbreviations: N, normal tissue; T, tumoral tissue.