Table 2.

Differential methylation in cord blood DNA in relation to the timing of the mother's smokinga

Quit before pregnancy vs. never smokersQuit during pregnancy by 18 weeks vs. never smokersSmoked through gestational week 18 vs. never smokers
ChrbGeneDistance to genecCpGPositiondCoefeSEfPCoefSEPCoefSEP
1GFI13,688cg10399789929456680.0010.0040.867−0.0080.0050.091−0.0410.0062.19E−10
1GFI13,224cg09662411929461320.0020.0050.654−0.0170.0070.016−0.0820.0097.47E−19
1GFI13,169cg0633871092946187−0.0020.0040.581−0.0110.0060.046−0.0630.0092.02E−13
1GFI12,656cg18146737929467000.0050.0060.439−0.0210.0080.011−0.1170.0124.21E−23
1GFI12,531cg12876356929468250.0060.0070.415−0.0200.0100.046−0.1340.0121.62E−27
1GFI12,321cg18316974929470350.0010.0030.810−0.0100.0040.016−0.0710.0082.93E−18
1GFI11,768cg09935388929475880.0100.0080.227−0.0250.0100.014−0.1490.0137.74E−32
1GFI11,395cg14179389929479610.0040.0070.568−0.0110.0080.155−0.0870.0072.07E−35
5AHRR19,617cg23067299323907−0.0020.0040.5690.0000.0041.0000.0300.0051.77E−09
5AHRR64,157cg03991871368447−0.0050.0020.0350.0000.0030.906−0.0210.0034.23E−10
5AHRR69,088cg05575921373378−0.0030.0030.343−0.0040.0040.302−0.0740.0089.70E−22
5AHRR95,070cg21161138399360−0.0060.0030.033−0.0040.0040.320−0.0310.0049.79E−13
6HLA-DPB211,549cg1171594333091841−0.0030.0030.4110.0030.0040.364−0.0190.0042.99E−06
7MYO1G16,417cg1908920145002287−0.0020.0020.4730.0010.0020.5620.0130.0024.08E−08
7MYO1G16,218cg2213278845002486−0.0020.0030.5090.0020.0030.5890.0230.0033.80E−14
7MYO1G15,968cg0418004645002736−0.0010.0040.7470.0010.0050.8960.0610.0071.39E−19
7MYO1G15,785cg1280306845002919−0.0020.0080.7680.0070.0080.3640.0840.0092.09E−20
7ENSG00000225718198,306cg0459867068697651−0.0070.0060.236−0.0140.0080.076−0.0450.0078.88E−10
7CNTNAP2854cg259495501458143060.0010.0010.327−0.0020.0010.129−0.0160.0011.01E−39
8EXT1−33,821cg03346806119157879−0.0040.0030.2140.0040.0040.322−0.0140.0041.64E−04
14TTC7B274,756cg1865502591008005−0.0020.0020.2660.0020.0020.297−0.0110.0035.31E−05
15CYP1A1−1,266cg0554965575019143−0.0040.0040.272−0.0040.0040.3380.0360.0062.63E−10
15CYP1A1−1,374cg2254904175019251−0.0120.0080.156−0.0110.0100.2780.0650.0111.57E−08
15CYP1A1−1,406cg1192401975019283−0.0040.0050.440−0.0050.0060.4330.0390.0061.43E−09
15CYP1A1−1,425cg1809247475019302−0.0070.0070.336−0.0110.0090.2380.0600.0102.05E−09
21RUNX11,746cg1247788036259241−0.0010.0040.8380.0040.0060.5280.0380.0089.25E−07
  • aN = 1,042 individuals analyzed. Categorized using a combination of mother's self-report and mother's plasma cotinine measured during pregnancy at approximately gestational week 18, where cotinine values above 56.8 nmol/L indicate smoking. All analyses adjusted for maternal age, maternal education, and parity.

  • bChromosome.

  • cDistance (nucleotides) from CpG to transcription start site of the nearest gene.

  • dChromosomal position based on National Center for Biotechnology Information human reference genome assembly Build 37.3.

  • eRegression coefficient.

  • fStandard error for regression coefficient. CpGs with P values reaching a Bonferroni-corrected statistical significance threshold of 0.05/26 = 0.0019 are noted in bold.