Table 1.

Primers and probes used in real-time PCR

GenePrimer/probePrimer/probe sequenceAnnealing/extensionSize, bpNotes
VIMForward primerTCGTTTCGAGGTTTTCGCGTTAGAGAC68°C217Primers from ref. (2)
Reverse primerCGACTAAAACTCGACCGACTCGCGA
Probe[6FAM]-CGGGAGTTAGT[+T]CGCGTTATCGTCGTCGTTT-[BHQ1]
ActinForward primerGGATAGGATAGTTTTATTTTTAG57°C217
Reverse primerATACAAAACTATACTCAACCAA
Probe5′-6FAM-ACCACCACCCAACACACAATAACAAACACA-BHQ1-3′Probe from ref. (8)

NOTE: [+N] denotes locked nucleic acid (LNA) bases.