Table 1.

Expression of CagA in Colombian H. pylori strains

StrainCagA expressionaNumber of AATAAGATA motifsbRegioncMLST typedHistopathology scoree
50011.00LowhpAfrica12.3
50041.4 ± 0.20LowhpAfrica12.3
50050.4 ± 0.20LowhpAfrica11.7
50071.4 ± 0.30LowhpAfrica12.0
50091.4 ± 0.21LowhpEurope1.7
50100.5 ± 0.30LowhpAfrica12.0
50161.8 ± 0.20LowhpAfrica13.5
50170.9 ± 0.30LowhpAfrica12.3
50181.6 ± 0.51LowhpAfrica13.5
50220.9 ± 0.30LowhpEurope4.5
50241.9 ± 0.10LowhpAfrica12.0
50281.1 ± 0.41LowhpEurope3.8
50322.3 ± 0.21LowhpEurope3.5
50383.2 ± 0.51LowhpEurope3.5
50433.7 ± 0.71LowhpEurope5.3
50512.4 ± 0.21LowhpAfrica12.0
50541.6 ± 0.60LowhpAfrica12.3
50564.0 ± 0.81HighhpEurope4.5
50572.4 ± 0.41HighhpEurope3.5
50682.4 ± 0.41HighhpEurope4.3
50693.5 ± 0.61HighhpEurope4.3
50720.7 ± 0.21HighhpEurope3.5
50745.1 ± 1.11HighhpEurope4.7
50800.5 ± 0.31HighhpEurope3.5
50824.4 ± 1.31HighhpEurope4.8
50864.8 ± 1.11HighhpEurope4.6
50901.0 ± 0.20HighhpEurope2.0
50911.9 ± 0.21HighhpEurope4.5
50933.5 ± 0.31HighhpEurope4.5
50952.0 ± 0.21HighhpEurope2.0
50961.5 ± 0.10HighhpEurope4.3
50971.0 ± 0.31HighhpEurope4.4
51002.2 ± 0.11HighhpEurope2.3
51044.7 ± 0.61HighhpEurope3.5
51122.2 ± 0.11HighhpEurope4.3
51140.9 ± 0.30HighhpEurope4.4
  • aCagA expression was analyzed by immunoblotting, and relative levels of expression were quantified by comparing the CagA signal intensity of each strain with that of a standard (CagA from H. pylori strain 5001). For each strain, the CagA signal was calculated for 3 independent biological samples, and the average of these values is presented. Values represent mean ± SEM.

  • bThe number of AATAAGATA motifs upstream of the ATG translation initiation site is shown.

  • cH. pylori strains were isolated from regions of Colombia with either high risk or low risk for the development of gastric cancer.

  • dMLST analysis (32) was previously used to characterize the ancestral origin of the H. pylori strains. hpAfrica1 denotes isolates classified as either West African (hspWAfrica) or South African (hspSAfrica).

  • eHistopathology scores describing the severity of lesions observed in gastric tissue from which the H. pylori strains were isolated.