Expression of CagA in Colombian H. pylori strains
| Strain | CagA expressiona | Number of AATAAGATA motifsb | Regionc | MLST typed | Histopathology scoree |
|---|---|---|---|---|---|
| 5001 | 1.0 | 0 | Low | hpAfrica1 | 2.3 |
| 5004 | 1.4 ± 0.2 | 0 | Low | hpAfrica1 | 2.3 |
| 5005 | 0.4 ± 0.2 | 0 | Low | hpAfrica1 | 1.7 |
| 5007 | 1.4 ± 0.3 | 0 | Low | hpAfrica1 | 2.0 |
| 5009 | 1.4 ± 0.2 | 1 | Low | hpEurope | 1.7 |
| 5010 | 0.5 ± 0.3 | 0 | Low | hpAfrica1 | 2.0 |
| 5016 | 1.8 ± 0.2 | 0 | Low | hpAfrica1 | 3.5 |
| 5017 | 0.9 ± 0.3 | 0 | Low | hpAfrica1 | 2.3 |
| 5018 | 1.6 ± 0.5 | 1 | Low | hpAfrica1 | 3.5 |
| 5022 | 0.9 ± 0.3 | 0 | Low | hpEurope | 4.5 |
| 5024 | 1.9 ± 0.1 | 0 | Low | hpAfrica1 | 2.0 |
| 5028 | 1.1 ± 0.4 | 1 | Low | hpEurope | 3.8 |
| 5032 | 2.3 ± 0.2 | 1 | Low | hpEurope | 3.5 |
| 5038 | 3.2 ± 0.5 | 1 | Low | hpEurope | 3.5 |
| 5043 | 3.7 ± 0.7 | 1 | Low | hpEurope | 5.3 |
| 5051 | 2.4 ± 0.2 | 1 | Low | hpAfrica1 | 2.0 |
| 5054 | 1.6 ± 0.6 | 0 | Low | hpAfrica1 | 2.3 |
| 5056 | 4.0 ± 0.8 | 1 | High | hpEurope | 4.5 |
| 5057 | 2.4 ± 0.4 | 1 | High | hpEurope | 3.5 |
| 5068 | 2.4 ± 0.4 | 1 | High | hpEurope | 4.3 |
| 5069 | 3.5 ± 0.6 | 1 | High | hpEurope | 4.3 |
| 5072 | 0.7 ± 0.2 | 1 | High | hpEurope | 3.5 |
| 5074 | 5.1 ± 1.1 | 1 | High | hpEurope | 4.7 |
| 5080 | 0.5 ± 0.3 | 1 | High | hpEurope | 3.5 |
| 5082 | 4.4 ± 1.3 | 1 | High | hpEurope | 4.8 |
| 5086 | 4.8 ± 1.1 | 1 | High | hpEurope | 4.6 |
| 5090 | 1.0 ± 0.2 | 0 | High | hpEurope | 2.0 |
| 5091 | 1.9 ± 0.2 | 1 | High | hpEurope | 4.5 |
| 5093 | 3.5 ± 0.3 | 1 | High | hpEurope | 4.5 |
| 5095 | 2.0 ± 0.2 | 1 | High | hpEurope | 2.0 |
| 5096 | 1.5 ± 0.1 | 0 | High | hpEurope | 4.3 |
| 5097 | 1.0 ± 0.3 | 1 | High | hpEurope | 4.4 |
| 5100 | 2.2 ± 0.1 | 1 | High | hpEurope | 2.3 |
| 5104 | 4.7 ± 0.6 | 1 | High | hpEurope | 3.5 |
| 5112 | 2.2 ± 0.1 | 1 | High | hpEurope | 4.3 |
| 5114 | 0.9 ± 0.3 | 0 | High | hpEurope | 4.4 |
↵aCagA expression was analyzed by immunoblotting, and relative levels of expression were quantified by comparing the CagA signal intensity of each strain with that of a standard (CagA from H. pylori strain 5001). For each strain, the CagA signal was calculated for 3 independent biological samples, and the average of these values is presented. Values represent mean ± SEM.
↵bThe number of AATAAGATA motifs upstream of the ATG translation initiation site is shown.
↵cH. pylori strains were isolated from regions of Colombia with either high risk or low risk for the development of gastric cancer.
↵dMLST analysis (32) was previously used to characterize the ancestral origin of the H. pylori strains. hpAfrica1 denotes isolates classified as either West African (hspWAfrica) or South African (hspSAfrica).
↵eHistopathology scores describing the severity of lesions observed in gastric tissue from which the H. pylori strains were isolated.