Table 2.

Summary of performance of multiplexed markers on Bio-Rad and Millipore

Bio-Rad markers with acceptable performance^a	Bio-Rad markers with <25% detectability^b	Millipore markers with acceptable performance	Millipore markers with acceptable performance^a	Millipore markers with <25% detectability^b
A2M	B_NGF	Amylin_Total	MIP_1D	Eotaxin 3
CRP	GM_CSF	BCA_1	PP	Ghrelin
CTACK	IFNA2	CCL19_MIP3B	PYY	IL_20
C Peptide	IL_12P40	CCL20_MIP3A	SAA	IL_21
Eotaxin	IL_15	CKINE	SAP	IL_28a
Ferritin	IL_1A	CRP	SCD30	IL_3
FGF_Basic	IL_2	CTACK	SCD40L	IL_4
Fibrinogen	IL_3	CXCL11_I_TAC	SCF	IL_5
Ghrelin	LIF	CXCL6_GCP2	SDF_1A	M_CSF
GIP	MCP_3	CXCL9_MIG	SEGFR	XCL1_Lympho
GLP_1	TNFB	C_Peptide	SGP130	Millipore markers
Glucagon		EGF	SILRII	with >20% CV^c
GRO	Bio-Rad markers	ENA_78	SIL_1RI
Haptoglobin	with >20% CV^c	Eotaxin	SIL_2RA	GM_CSF
HGF		Eotaxin_2	SIL_4R	IL_10
IFNG	B_NGF	FGF_Basic	SIL_6R	IL_12P70
IL_16	GM_CSF	FIT_3_Ligand	SRAGE	IL_13
IL_18	G_CSF	Fractalkine	STNFRI	IL_15
IL 1RA	IL_10	GIP	STNFRII	IL_17
IL 2RA	IL_12P70	GLP_1	SVEGFR1	IL_1B
IL_6	IL_13	Glucagon	SVEGFR2	IL_1RA
IL_8	IL_15	GRO	SVEGFR3	IL_2
IL 9	IL_17	G_CSF	TARC	IL_21
Insulin	IL_1A	IFNA2	TNF-α	IL_23
IP_10	IL 1B	IFNG	TPO	IL_28A
Leptin	IL_2	IL_11	TRAIL	IL_3
MCP_1MC	IL_4	IL_12P40	TSLP	IL_4
MIF	IL_5	IL_16	VEGF	IL_5
MIG	IL_7	IL_1A		IL_6
MIP_1B	LIF	IL_29_IFNG1		IL_7
M_CSF	MCP_3	IL_33		IL_9
PAI_1	MIP 1A	IL_8		I_309
PCT	TNF_B	INSULIN		M_CSF
PDGF_BB	TPA	IP_10		TGFA
Rantes		LEPTIN		TNF-B
Resistin		LIF		XCL1_Lympho
SAA		MCP_1
SAP		MCP_2
SCF		MCP_3
SCGF_B		MCP_4
SDF_1A		MDC
TNF-α		MIP_1A
TRAIL		MIP_1B
VEGF
Visfatin

^aAcceptable performance was defined as (i) being detectable in greater than 25% of the 100 samples on all 3 specimen types and (ii) across-batch CVs of less than 20% for blinded duplicates on at least 2 of the 3 specimen types.
^bMarkers with less than 25% detectability on at least 1 of 3 (serum, heparin plasma, and EDTA plasma) specimen types.
^cMarkers with CVs for across-batch duplicates greater than 20% on 2 or more of 3 specimen types. CVs were calculated for 20 blinded duplicate samples for each specimen type that were placed across different batches.