Table 2

Interrun variation and normalization

SampleUrine BBI concentration (ng/ml) determined in six experimentsa
123456MeanSDCV
Before normalization
123.8424.0717.1323.8221.2930.0023.364.200.180
218.614.3912.3518.4919.7925.3618.164.530.250
328.4322.6019.0431.8439.6530.5528.697.260.253
412.6611.269.5514.0019.9424.8614.555.400.372
519.4314.4114.4217.5318.5727.2418.574.760.256
Av.20.5917.3514.4621.1322.8527.6020.665.230.253
Normalization ratiob
1.0031.1911.4290.9780.9040.7491.000
After normalizationc
123.9628.6724.4823.2919.2622.4623.683.060.129
218.6717.1417.6518.0817.9018.9918.070.670.037
328.5326.9327.2231.1335.8622.8728.764.390.153
412.7113.4113.6613.6813.5118.6114.262.160.151
519.5017.1720.3117.1416.8020.3918.551.700.091
Average20.6620.6620.6620.6620.6620.6620.662.400.112
  • a The BBI concentrations were measured six times in a set of five urine samples by the ELISA-C method. The mean, SD, and coefficient of variation (CV) represent the data from six independent experiments (n = 6).

  • b The normalization ratios were calculated by dividing the average urine BBI concentration (20.66 ng/ml) for the five urine samples in six experiments by the average urine BBI concentration for the same urine samples measured in each experiment.

  • c The BBI concentrations of the urine samples measured in each experiment were normalized with the respective normalization ratio.