Table 3

DNA yields and PCR amplification results from five buccal cell samples collected on treated cards and stored under various conditions

Sample typenDNA yield (μg)n (%) amplified 536-bp fragment
Card ACard BCard CCard DCard E
Fresh51.01.93.17.545.65 (100)
Stored 9 mo
Room temperaturea50.41.81.53.917.85 (100)
−20°Cb50.71.73.92.018.35 (100)
−70°Cc50.90.51.81.49.65 (100)
  • a P = 0.01, paired t test of standardized difference in DNA yields, room temperature storage versus fresh.

  • b P = 0.17, paired t test of standardized difference in DNA yields, −20°C storage versus fresh.

  • c P = 0.02, paired t test of standardized difference in DNA yields, −70°C storage versus fresh.