Table 3

Effect of eight phenolic constituents of brown rice on proliferation of breast- and colon-derived cells as assessed by the MTS assay

Breast cell linesColon cell lines
MDA MB468MCF7HBL100HT29SW480HCEC
Protocatechuic acid97.0a107.1131.8b102.1110.195.9
Caffeic acid83.6b72.9b96.671.9b59.6b79.4b
p-Coumaric acid100.0109.3100.086.5111.286.6
Vanillic acid94.092.1117.1b81.3102.285.6
Methoxycinnamic acid94.0109.393.264.6b113.380.4b
Ferulic acid97.089.3110.295.8105.675.3b
Sinapic acid104.4102.9121.6b106.3117.3b88.7
Tricin65.7b104.377.3b55.2b104.584.5
Genistein62.7b60.0b72.7b58.3b77.5b80.4b
  • a Results (mean of three to five observations) are presented as percentages of viable cell numbers in control incubations (omitting phenols) as reflected by the respective absorbance units (490 nm). Phenols were applied at 50μ m; 30 μm genistein served as a positive control. The mean and SD of the absorbance units of each data set relating to a particular cell line were calculated by the ANOVA General Linear Model. Mean absorbances in control incubations were 0.67, 1.40, 0.88, 0.96, 0.89, and 0.97 in MDA MB468, MCF7, HBL 100, HT29, SW480, and HCEC cells, respectively; the SD of each set varied between 0.08 (MDA MB468 cells) and 0.26 (MCF7 cells) absorbance units. For details of cell culture and experimental design, see “Materials and Methods.”

  • b Values are significantly different from those for control cells (P < 0.05).