Breast cell lines | Colon cell lines | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
MDA MB468 | MCF7 | HBL100 | HT29 | SW480 | HCEC | |||||
Extract | 27.3^{a},^{b} | 90.4 | 22.0^{b} | 76.8 | 37.6^{b} | 36.4^{b} | ||||
Fraction 1 | 39.4^{b} | 34.6^{b} | 81.7^{b} | 101.4 | 80.0^{b} | 85.2 | ||||
Fraction 2 | 90.9 | 93.3 | 88.1 | 114.4 | 125.9^{b} | 120.5^{b} | ||||
Fraction 3 | 101.5 | 95.2 | 85.3^{b} | 123.8^{b} | 122.3^{b} | 105.7 | ||||
Fraction 4 | 112.1 | 109.6 | 90.8 | 134.8^{b} | 118.8^{b} | 129.5^{b} | ||||
Fraction 5 | 110.6 | 104.8 | 107.3 | 123.2 | 138.8^{b} | 125.0^{b} | ||||
Fraction 6 | 107.6 | 99.0 | 96.3 | 117.4 | 144.7^{b} | 129.5^{b} | ||||
Fraction 7 | 98.5 | 94.2 | 94.5 | 143.5^{b} | 128.2^{b} | 121.6^{b} | ||||
Fraction 8 | 59.1^{b} | 27.9^{b} | 57.8^{b} | 71.0^{b} | 56.5^{b} | 62.5^{b} | ||||
Fraction 9 | 48.5^{b} | 32.7^{b} | 51.4^{b} | 71.0^{b} | 58.8^{b} | 56.8^{b} | ||||
Genistein | 47.0^{b} | 60.6^{b} | 69.7^{b} | 66.7^{b} | 65.9^{b} | 72.7^{b} |
^{a} Results (mean of three to five observations) are presented as percentages of viable cell numbers in control incubations (omitting extracts or fractions) as reflected by the respective absorbance units (490 nm). Extract and fractions were applied at 100 μg/ml; 30 μm genistein served as a positive control. The mean and SD of the absorbance units of each set relating to a particular cell line were calculated by the ANOVA General Linear Model. Mean absorbances in control incubations were 066, 1.04, 1.09, 0.69, 0.85, and 0.88 in MDA MB468, MCF7, HBL 100, HT29, SW480, and HCEC cells, respectively; the SD of each set varied between 0.11 (MDA MB468 cells) and 0.16 (MCF7 cells) absorbance units. For details of cell culture and experimental design, see “Materials and Methods.”
^{b} Absorbance values are significantly different from those for control cells (P < 0.05).