Table 2

Effect of ethyl acetate extract of rice bran and chromatographic fractions 1–9 on proliferation of breast- and colon-derived cells as assessed by the MTS assay

Breast cell linesColon cell lines
MDA MB468MCF7HBL100HT29SW480HCEC
Extract27.3a,b90.422.0b76.837.6b36.4b
Fraction 139.4b34.6b81.7b101.480.0b85.2
Fraction 290.993.388.1114.4125.9b120.5b
Fraction 3101.595.285.3b123.8b122.3b105.7
Fraction 4112.1109.690.8134.8b118.8b129.5b
Fraction 5110.6104.8107.3123.2138.8b125.0b
Fraction 6107.699.096.3117.4144.7b129.5b
Fraction 798.594.294.5143.5b128.2b121.6b
Fraction 859.1b27.9b57.8b71.0b56.5b62.5b
Fraction 948.5b32.7b51.4b71.0b58.8b56.8b
Genistein47.0b60.6b69.7b66.7b65.9b72.7b
  • a Results (mean of three to five observations) are presented as percentages of viable cell numbers in control incubations (omitting extracts or fractions) as reflected by the respective absorbance units (490 nm). Extract and fractions were applied at 100 μg/ml; 30 μm genistein served as a positive control. The mean and SD of the absorbance units of each set relating to a particular cell line were calculated by the ANOVA General Linear Model. Mean absorbances in control incubations were 066, 1.04, 1.09, 0.69, 0.85, and 0.88 in MDA MB468, MCF7, HBL 100, HT29, SW480, and HCEC cells, respectively; the SD of each set varied between 0.11 (MDA MB468 cells) and 0.16 (MCF7 cells) absorbance units. For details of cell culture and experimental design, see “Materials and Methods.”

  • b Absorbance values are significantly different from those for control cells (P < 0.05).