Table 3.

Serum fatty acids by p53 immunoreactivity expressed as proportion of p53-positive cells in the basal layer of the epidermis (mean and 95% CI) by tertile group of serum fatty acids; Nambour Skin Cancer Prevention Trial, Australia Prevention Trial, Australia

Fatty acidTertile 1Tertile 2Tertile 3Pa
Total n-3b, μg/mL36.7 (20.7–43.6)50.2 (43.8–55.8)67.1 (55.9–112.2)
 % Basicc11.2 (7.3–17.1)7.7 (5.3–11.3)5.8 (4.1–8.3)0.04
 % Multivariabled13.0 (7.4–22.7)7.4 (4.4–12.5)5.8 (3.7–9.2)0.008
ALA, μg/mL1.2 (0.5–1.5)1.8 (1.5–2.2)2.9 (2.2–5.2)
 % Basic6.8 (4.5–10.3)6.6 (4.5–9.6)8.8 (6.0–12.8)0.44
 % Multivariable6.1 (3.5–10.7)6.4 (3.8–10.9)8.1 (4.9–13.2)0.53
EPA, μg/mL6.5 (2.7–8.0)10.1 (8.1–12.2)14.8 (12.3–32.6)
 % Basic8.7 (5.7–13.4)8.4 (5.7–12.5)6.3 (4.3–9)0.38
 % Multivariable9.3 (5.3–16.0)7.7 (4.6–12.8)5.8 (3.5–9.6)0.21
DHA, μg/mL26.7 (16.0–31.7)37.7 (32.0–42.1)52.1 (42.2–77.4)
 % Basic10.6 (7.0–16.1)6.5 (4.5–9.5)6.8 (4.8–9.7)0.08
 % Multivariable13.0 (7.4–23.0)6.5 (4.0–10.7)6.5 (4.0–10.4)0.009
Total n-6, μg/mL305.5 (187.1–331.1)361.7 (331.6–387.3)426.6 (389.4–573.7)
 % Basic7.3 (5.0–10.8)9.3 (6.3–13.8)6.0 (4.1–8.8)0.20
 % Multivariable8.2 (5.0–13.6)8.5 (4.9–14.8)5.6 (3.3–9.2)0.19
LA, μg/mL194.3 (122.8–212.7)240.3 (214.1–264.2)290.2 (267.2–413.4)
 % Basic7.4 (5.0–10.9)9.6 (6.4–14.4)6.2 (4.3–9.0)0.23
 % Multivariable8.1 (4.8–13.6)7.8 (4.4–13.8)6.2 (3.8–10.2)0.54
AA, μg/mL98.3 (54.8–114.7)124.4 (115.3–132.2)148.1 (134.1–181.8)
 % Basic7.9 (5.4–11.5)8.7 (5.9–12.9)6.2 (4.2–9.0)0.33
 % Multivariable8.4 (5.0–14.0)8.4 (5.0–14.1)5.5 (3.3–9.0)0.15
Ratio n-3/n-60.10 (0.08–0.13)0.14 (0.13–0.15)0.18 (0.15–0.38)
 % Basic8.4 (5.5–12.9)8.0 (5.4–11.8)6.7 (4.7–9.5)0.62
 % Multivariable9.1 (5.1–16.1)7.2 (4.3–12.0)6.4 (3.9–10.6)0.41

Abbreviations: AA, arachidonic acid; ALA, alpha-linolenic acid; LA, linoleic acid.

  • aFrom ANOVA for unbalanced design, type III sum of squares.

  • bMedian (minimum–maximum), all such values.

  • cProportion of p53-positive cells (mean and 95% CI) adjusted for age and sex.

  • dProportion of p53-positive cells (mean and 95% CI) adjusted for age, sex, skin color, propensity to sunburn, β-carotene allocation during the trial, and use of sunscreen on the hands.