Table 1.

Comparison of O6-meG levels in methylated DNA samples prepared using [3H]-MNU and analyzed by HPLC/scintillation counting or the new ELISA method

Mean O6-meG value measured by HPLCO6-meG measured by ELISA (same-day triplicates), adducts/108 nucleotidesMean (SD) ELISA valueMean ELISA value as % of HPLC value
26.122.4, 24.2, 21.922.8 (1.2)87.4
35.530.3, 35.5, 37.234.3 (3.6)96.6