Table 2.

Correlation between mRNA expression levels and immunohistochemical staining levels as measured by pathologists and automated systems

Stainρ (95% CI)P
ER-α
    Pathologist0.67 (0.45-0.89)4 × 10−12
    Aperio0.73 (0.51-0.95)4 × 10−15
    TMAx0.74 (0.52-0.96)1 × 10−15
    Ariol0.67 (0.45-0.89)2 × 10−12
PR
    Pathologist0.77 (0.55-0.99)6 × 10−17
    Aperio0.69 (0.47-0.91)6 × 10−13
    TMAx0.73 (0.51-0.95)1 × 10−14
    Ariol0.73 (0.51-0.95)5 × 10−15
HER2*
    Pathologist0.63 (0.41-0.85)4 × 10−10
    Aperio0.43 (0.21-0.65)1 × 10−4
    TMAx0.49 (0.27-0.71)4 × 10−6
    Ariol0.41 (0.19-0.63)2 × 10−4
ER-β
    Pathologist−0.04 (−0.22 to 0.22)0.76
    Aperio0.09 (−0.13 to 0.31)0.43
    TMAx0.003 (−0.22 to 0.22)0.97
    Ariol−0.12 (−0.34 to 0.1)0.3
Aromatase*
    Pathologist0.06 (−0.17 to 0.29)0.61
    Aperio−0.03 (−0.26 to 0.2)0.79
    TMAx0.04 (−0.19 to 0.27)0.76
    Ariol0.09 (−0.14 to 0.32)0.44

NOTE: For ER-α, PR, and ER-β, the combined (intensity × percent) score was used. For HER2 and aromatase, the intensity score was used.

  • *Results are for the expression probe with the highest correlation coefficients.