Table 1.

TMAs: selected design considerations

Design considerationProsCons
Increased cores/sampleReduces missing dataIncreases effort
Captures heterogeneity
Larger core diameterBetter target representationFewer cores/TMA
Replicate cores separated in different TMAsProtects material if one TMA block lostNeed to ensure consistency
More cores/TMAReduces assay costsMapping more difficult
Grouping cores within TMA blocks by subject or tumor characteristicsConserves material for relevant assaysNeed to ensure consistency