Table 2.

Preanalytical variables included in the SPREC (seven-element-long SPREC), version SPREC-01, applied to solid samples. Codes in bold come from the LDMS

Type of sample
    Fresh cells from nonblood specimen typeCEN
    Cells from nonblood specimen type (e.g., disrupted tissue), viableCLN
Cells from fine needle aspirateFNA
    HairHAR
    Cells from laser capture microdissected tissue LCM
    Cells from nonblood specimen type (e.g., disrupted tissue), nonviablePEN
    Solid tissueTIS
    Cells from disrupted tissueLCM
    OtherZZZ
Type of collection
    Autopsy <6 h postmortemA06
    Autopsy 6-12 h postmortemA12
    Autopsy 12-24 h postmortemA24
    Autopsy 24-48 h postmortemA48
    Autopsy 48-72 h postmortemA72
    BiopsyBPS
    Fine needle aspirateFNA
    PunctionPUN
    Surgical excisionSRG
    SwabSWB
    OtherZZZ
Warm ischemia time
    <2 minA
    2-10 minB
    10-20 minC
    20-30 minD
    30-60 minE
    >60 minF
    UnknownX
    Not applicable (e.g., biopsy)N
    OtherZ
Cold ischemia time
    <2 minA
    2-10 minB
    10-20 minC
    20-30 minD
    30-60 minE
    >60 minF
    UnknownX
    Not applicable (e.g., autopsy)N
    OtherZ
Fixation type
    Nonaldehyde with acetic acidACA
    Aldehyde basedALD
    Alcohol basedETH
    Nonbuffered formalinFOR
    Snap freezingSNP
    Nonaldehyde without acetic acidNAA
    Neutral buffered formalinNBF
    Optimum cutting temperature mediumOCT
    RNA LaterRNL
    UnknownXXX
    OtherZZZ
Fixation time
    <15 minA
    15 min to 1 hB
    1-4 hC
    4-8 hD
    8-24 hE
    24-48 hF
    48-72 hG
    UnknownX
    OtherZ
Long-term storage
    PP tube 0.5-2 mL*−85°C to −60°CA
    PP tube 0.5-2 mL−35°C to −18)°CB
    Cryotube 1-2 mLLiquid nitrogenC
    Cryotube 1-2 mL−85°C to −60°CD
    Cryotube 1-2 mLProgrammable freezing to <−135°CE
    StrawLiquid nitrogenF
    Straw−85°C to −60°CG
    Straw−35°C to −18°CH
    StrawProgrammable freezing to <−135°CI
    PP tube ≥5 mL−85°C to −60°CJ
    PP tube ≥5 mL−35°C to −18°CK
    Microplate−85°C to −60°CL
    Microplate−35°C to −18°CM
    Paraffin blockRTP
    UnknownX
    OtherZ

NOTE: RT, room temperature: 18°C to 25°C.

  • *PP, polypropylene.

  • Liquid nitrogen refers to either vapor or liquid phase.