Table 1.

Preanalytical variables included in the SPREC (seven-element-long SPREC), version SPREC-01, applied to fluid samples. Codes in bold come from the LDMS

Type of sample
    Ascites fluidASC
    Amniotic fluidAMN
    Bronchoalveolar lavageBAL
    Blood (whole)BLD
    Bone marrow aspirateBMA
    Breast milkBMK
    Buccal cellsBUC
    Unficolled buffy coat, viableBUF
    Ficoll mononuclear cells, viableCEL
    Fresh cells from non blood specimen typeCEN
    Cells from nonblood specimen type (e.g., disrupted tissue), viableCLN
    Cord bloodCRD
    Cerebrospinal fluidCSF
    Nasal washingNAS
    Ficoll mononuclear cells, nonviablePEL
    Cells from nonblood specimen type (e.g., disrupted tissue), nonviablePEN
    Pleural fluidPFL
    Plasma, single spunPL1
    Plasma, double spunPL2
    SalivaSAL
    SemenSEM
    SerumSER
    SputumSPT
    StoolSTL
    Synovial fluidSYN
    TearsTER
    24-h urineU24
    UrineURN
    OtherZZZ
Type of primary container
    Vacutainer acid citrate dextrose or equivalentACD
    Vacutainer citrate phosphate dextrose or equivalentCPD
    Vacutainer lithium heparin or equivalentHEP
    Vacutainer hirudin or equivalentHIR
    Oragene collection container or equivalentORG
    Paxgene blood RNA+PAX
    Vacutainer potassium EDTA or equivalentPED
    S8820 protease inhibitor tablets or equivalentPI1
    Protease inhibitorsPIX
    Polypropylene tube sterile PPS
    Paxgene blood DNAPXD
    Paxgene bone marrow RNAPXR
    Vacutainer sodium citrate or equivalentSCI
    Vacutainer sodium EDTA or equivalentSED
    Vacutainer sodium fluoride/potassium oxalate or equivalentSPO
    Vacutainer serum separator tube or equivalentSST
    Tempus tubeTEM
    Vacutainer trace elementsTRC
    UnknownXXX
    OtherZZZ
    Precentrifugation (delay between collection and processing)
    RT*<2 hA
    3°C to 7°C<2 hB
    RT2-4 hC
    3°C to 7°C2-4 hD
    RT4-8 hE
    3°C to 7°C4-8 hF
    RT8-12 hG
    3°C to 7°C8-12 hH
    RT12-24 hI
    3°C to 7°C12-24 hJ
    RT24-48 hK
    3°C to 7°C24-48 hL
    RT>48 hM
    3°C to 7°C>48 hN
    35°C to 38°C<2 hO
    UnknownX
    OtherZ
Centrifugation
    RT 10 min<3,000 g no brakingA
    RT 10 min<3,000 g with brakingB
    3°C to 7°C 10 min<3,000 g no brakingC
    3°C to 7°C 10 min<3,000 g with brakingD
    RT 10 min3,000-6,000 g with brakingE
    3°C to 7°C 10 min3,000-6,000 g with brakingF
    RT 10 min6,000-10,000 g with brakingG
    3°C to 7°C 10 min6,000-10,000 g with brakingH
    RT 10 min>10,000 g with brakingI
    3°C to 7°C 10 min>10,000 g with brakingJ
    No centrifugationN
    UnknownX
    OtherZ
Second centrifugation
    RT 10 min<3,000 g no brakingA
    RT 10 min<3,000 g with brakingB
    3°C to 7°C 10 min<3,000 g no brakingC
    3°C to 7°C 10 min<3,000 g with brakingD
    RT 10 min3,000-6,000 g with brakingE
    3°C to 7°C 10 min3,000-6,000 g with brakingF
    RT 10 min6,000-10,000g with brakingG
    3-7°C 10 min6,000-10,000 g with brakingH
    RT 10 min>10,000 g with brakingI
    3°C to 7°C 10 min>10,000 g with brakingJ
    No second centrifugationN
    UnknownX
    OtherZ
Postcentrifugation delay
    <1 h 3°C to 7°CA
    <1 h RTB
    1-2 h 3°C to 7°CC
    1-2 h RTD
    2-8 h 3°C to 7°CE
    2-8 h RTF
    8-24 h 3°C to 7°CG
    8-24 h RTH
    >24 h 3°C to 7°CI
    >24 h RTJ
    UnknownX
    OtherZ
Long-term storage
    PP tube 0.5-2 mL−85°C to −60°CA
    PP tube 0.5-2 mL−35°C to −18°CB
    Cryotube 1-2 mLLiquid nitrogenC
    Cryotube 1-2 mL−85°C to −60°CD
    Cryotube 1-2 mLProgrammable freezing to <−135°CE
    StrawLiquid nitrogenF
    Straw−85°C to −60°CG
    Straw−35°C to −18°CH
    StrawProgrammable freezing to <−135°CI
    PP tube ≥5 mL−85°C to −60°CJ
    PP tube ≥5 mL−35°C to −18°CK
    Microplate−85°C to −60°CL
    Microplate−35°C to −18°CM
    Paraffin blockRTP
    UnknownX
    OtherZ
  • *RT, room temperature: 18°C to 25°C.

  • PP, polypropylene.

  • Liquid nitrogen refers to either vapor or liquid phase.