Table 1.

List of identified tumor antigens by MALDI-TOF

SpotProtein nameAccession no.Isoelectric point
MW (Da)
Mascot ScoreSequence coverage (%)Immuno-reactivity
Theor.Observ.Theor.Observ.
S1Kininogen-1 precursorP010426.345.3272,98465,43210624Normal sera
S2Kininogen-1 precursorP010426.345.2872,98467,65413224Normal sera
S3Kininogen-1 precursorP010426.345.2272,98469,22311334Normal sera
S4α-2-HS-glycoprotein precursorP027655.435.1040,09849,9986113Cancer sera
S5α-2-HS-glycoprotein precursorP027655.435.1340,09848,1007522Cancer sera
S6α-2-HS-glycoprotein precursorP027655.435.1640,09847,8757020Cancer sera
S7Monocyte differentiation antigen CD14P085715.846.2240,67868,3966120Normal sera
S8Zinc-α-2-glycoprotein precursorP253115.575.6134,07938,5606829Cancer sera
S9Cathepsin D precursorP073396.106.0745,03745,2017540Cancer sera
S10Cathepsin D precursorP073396.106.2145,03744,89810042Cancer sera
S11Clusterin precursorP109095.895.4953,03134,3157430Cancer sera
S12Ig α-2 chain C regionP018775.716.6937,28357,4356324Normal sera
S13Ig α-2 chain C regionP018775.716.8737,28356,9986416Normal sera
  • NOTE: The calculation of experimental isoelectric point and MW was based on migration of the protein spot on two-dimensional electrophoresis gels.

    Abbreviations: Theor., theoretical; Observ., observed.