Table 8.

Standard options for Biospecimen Research protocol applied to solid tissues

Type of fixation:Preanalytical variable
PrefixationWarm ischemia time: 0, less than or equal to 10 min*, 15-20 min, 25-30 min, 50-60 min
Cold ischemia time (delay time 1 (Δt1) before fixation): 2 min, 15 min, 30 min*, 60 min, 120 min
FixationCryopreservationSnap freezing*, OCT embedding
No cryopreservationBuffered formalin*, methanol/chloroform/acetone-based, aldehyde-based, nonaldehyde-based/without acetic acid, nonaldehyde-based/with acetic acid
PostfixationDelay time 2 (Δt2) from OCT embedding to freezing: 0, 15 min, 4-8 h*, 24 h, 48 h, 72 h
Storage temperature (for cryopreservation): −80°C*, vapor-phase liquid nitrogen
Storage duration (years): 1*, 3, 6, 9, 12, n
  • Abbreviations: Δt, delay, OCT, optimal cutting temperature.

  • * Baseline processing of the “reference” solid tissue sample.