Table 6.

Standard options for Biospecimen Research protocol applied to urine

Preanalytical variable
PrecentrifugationType of collection: first void, later void, 12 h, 24 h*
Type of container: with protease inhibitors, without protease inhibitors*
Delay time 1 (Δt1): less than 2 h*, 2 h, 4 h, 8 h, 24 h, 48 h
Temperature: 20°C-25°C, 3°C-7°C*, 35°C-38°C, −80°C
CentrifugationTemperature: 20°C-22°C*, 3°C-7°C
Force, delay time 2 (Δt2): 1,000 × g 10 min, 2,000 × g 10 min*, 4,000 × g 10 min
Number of centrifugations: 1, 2*
PostcentrifugationSecondary container: PP microtube*, plastic straw, PP 5-ml tube
Delay time 3 (Δt3): less than 2 h*, 2 h, 4 h, 8 h, 24 h, 48 h, 72 h
Prealiquoting temperature: 22°C-25°C, 3°C-7°C*, 35°C-38°C
Storage temperature: −20°C, −80°C*, vapor-phase liquid nitrogen
Number of freeze-thaws: 1*, 2, 4, 10, 20, 30
Storage duration (years): 1*, 3, 6, 9, 12, n
  • Abbreviations: Δt, delay; pp, polypropylene.

  • * Baseline processing of the “reference” urine sample.