Table 5.

Standard options for Biospecimen Research protocol applied to plasma

Preanalytical variable
PrecentrifugationType of tube: EDTA*, heparin, ACD, citrate, sodium fluoride/sodium oxalate, micro tube with gel
Delay time 1 (Δt1): less than 2 h*, 2 h, 4 h, 8 h, 24 h, 48 h, 72 h
Temperature: 20°C-25°C, 3°C-7°C*, 35°C-38°C
CentrifugationTemperature: 20°C-22°C*, 3°C-7°C
Number of centrifugations: 1, 2*
PostcentrifugationSecondary container: PP tube*, plastic straw, glass vial
Delay time 2 (Δt2): <1 h*, 2 h, 4 h, 8 h, 24 h, 48 h, 72 h
Prealiquoting temperature: 22°C-25°C, 3°C-7°C*, 35°C-38°C
Storage temperature: −20°C, −80°C*, vapor-phase liquid nitrogen
Number of freeze-thaws: 1*, 2, 4, 10, 20, 30
Storage duration (years): 1.5*, 3, 6, 9, 12, n
  • Abbreviations: Δt, delay; pp, polypropylene.

  • * Baseline processing of the “reference” plasma sample.