Table 2.

Constant conditions to be used for serum samples

Serum-related preanalytical conditions
PrecentrifugationCollection (primary) tube, fully filled
Collection tube, inverted 5-10 times
Δt coagulation, following manufacturer's instructions (30-60 min or 15-30 min)
Collection tube, kept closed
Collection tube maintained in vertical position
CentrifugationNo fixed angle rotor
No recentrifugation
2,000 × g for 10 min (or according to manufacturer's instructions)
PostcentrifugationNo inactivation (56°C)
No storage at RT, 4°C
  • Abbreviations: RT, room temperature; Δt, delay.