Table 2.

Estrogen receptor α methylation (%) in normal colonic tissue and leukocytes by CpG site and the average across all sites (methylation index) in subjects by disease status

SiteDisease free (n = 57)
Cancer (n = 27)
Adenoma (n = 30)
Hyperplastic (n = 16)
Colonic*LeukocyteColonicLeukocyteColonicLeukocyteColonicLeukocyte
18.7 (0.0-29.6)3.7 (0.0-17.4)11.4 (0.0-41.1)3.3 (0.0-10.4)14.2 (3.5-28.0)3.1 (0.0-8.8)11.9 (4.8-28.0)3.1 (0.0-9.1)
28.6 (0.0-26.0)4.8 (0.0-30.5)9.4 (0.0-41.5)6.1 (3.7-23.0)14.3 (5.5-26.5)4.7 (0.0-16.0)12.1 (7.7-27.1)4.1 (2.9-18.4)
35.3 (0.0-23.0)3.0 (0.0-12.9)8.2 (0.0-33.0)2.5 (0.0-7.5)11.1 (3.0-25.0)3.0 (0.0-12.5)10.0 (5.0-24.0)2.3 (0.0-4.3)
48.4 (0.0-30.2)5.2 (0.0-14.7)11.1 (0.0-39.9)5.2 (0.0-11.3)15.8 (5.6-29.8)5.1 (0.0-9.7)13.7 (7.2-28.7)4.8 (0.0-8.2)
58.9 (0.0-31.0)5.3 (0.0-14.7)11.5 (0.0-43.0)5.5 (0.0-17.7)17.0 (6.0-30.0)5.1 (0.0-13.9)14.1 (7.0-31.0)4.5 (0.0-9.1)
67.0 (0.0-27.4)5.7 (0.0-18.3)9.0 (0.0-39.3)7.0 (0.0-15.4)16.8 (6.8-30.3)5.9 (0.0-15.6)14.2 (7.8-29.9)5.4 (0.0-8.9)
75.8 (0.0-23.9)2.7 (0.0-14.7)7.7 (0.0-33.6)3.3 (0.0-14.5)10.2 (3.7-21.8)2.9 (0.0-11.0)9.3 (4.4-22.3)2.7 (0.0-12.5)
MI7.2 (1.0-27.2)4.1 (0.0-12.6)9.6 (0.8-38.7)4.8 (1.9-10.9)14.3 (4.9-27.2)4.2 (0.0-7.8)12.1§ (6.3-27.2)3.9 (2.7-6.2)
  • NOTE: All values are median (range).

    Abbreviation: MI, methylation index.

  • * n = 55.

  • n = 25.

  • P < 0.001, significantly different from disease-free group in same tissue (Kruskall-Wallis test followed by Mann Whitney U test with Bonferroni correction for multiple comparisons).

  • § P = 0.02, significantly different from disease-free group in same tissue (Kruskall-Wallis test followed by Mann Whitney U test with Bonferroni correction for multiple comparisons).