Table 9.

Cell cycle assays by flow cytometry for cigarette smoke

Cigarette Studies
Cell ModelMachine protocolCigarette typeTest substance*Cell cycle effects as reportedG0-G1 phaseS phaseG2-MReference
Human normal breast epithelial cell line MCF10AFTC1R4FCSC (Cambridge filter)Dose-dependent response observedDecreases compared with control at higher concentrationsIncrease compared with controlIncrease compared with controlNarayan, S 2004 (21)
Normal human bronchial epithelial cellsFTCKentucky 1R4F, EclipseTPM (Cambridge filter)Dose-dependent response observed for 1R4F, but not for EclipseDecline for 1R4F, no change for EclipseIncrease 1R4F, no change for EclipseIncrease for 1R4F, no change for EclipseFields, WR 2005 (155)
RAT-1 immortalized fibroblastsISON/ATPM (glass fiber filter)G0-G1 and S affected, but not G2MIncreasedDecreasedUnchangedPalozza, P 2005 (153)
RAT-1 immortalized fibroblastsISON/ATPM (glass fiber filter)G0/ G1 and S affected, but not G2MIncreasedDecreasedUnchangedPalozza, P 2006 (154)
Human lung epithelial cell line A549N/AIndian cigarette with filterCSC (Solvent trap)G0-G1 and S affected, but not G2-MIncreasedIncreased with lower CSC, decreased at highest concentrationUnchangedKaushik, G 2008 (76)
Normal human bronchial epithelial cellsFTCQuest cigarette low nicotine, nicotine free and 2R4FTPM (Cambridge filter)Dose-dependent response observed fIncreased at 16 h, decreased with both products 48 hDecreased at 16 h, Increased with both products 48 hDecreased at 16 h, Increased with both products 48 hChen, J 2008 (146)
Human lung epithelial cells A549N/ACommercial filtered cigaretteAECS (Solvent trap)Time- and dose-dependent inhibition of cell cycle progression observedSub-G0-G1 increased as G0-G1 decreased in a dose- and time-dependent mannerN/AN/ADas, A 2009 (152)
  • *TPM or CSC indicated as reported in publication, but actual method shown in parenthesis.