Table 1.

Primers and annealing temperature used for PCR

GenesM/US/ASPrimer sequences (5′→3′)Annealing temperature (°C)Product size (bp)
P16MSTTATTAGAGGGTGGGGCGGATCGC68150
ASGACCCCGAACCGCGACCGTAA
USTTATTAGAGGGTGGGGTGGATTGT60151
ASCAACCCCAAACCACAACCATAA
MGMTMSTTTCGACGTTCGTAGGTTTTCGC6681
ASGCACTCTTCCGAAAACGAAACG
USTTTGTGTTTTGATGTTTGTAGGTTTTTGT6093
ASAACTCCACACTCTTCCAAAAACAAAACA
hMLH1MSACGTAGACGTTTTATTAGGGTCGC60124
ASCCTCATCGTAACTACCCGCG
USTTTTGATGTAGATGTTTTATTAGGGTTGT60112
ASACCACCTCATCATAACTACCCACA
MTHFRSTGAAGGAGAAGGTGTCTGCGGGA61198
ASAGGACGGTGCGGTGAGAGTG
  • Abbreviations: M, methylated; U, unmethylated; S, sense; AS, antisense.