Primers and conditions for PCR
| SNP | DbSNP ID | SNP region | Amino acid change | PCR and pyrosequencing primers | PCR conditions |
|---|---|---|---|---|---|
| A1298C | rs1801131 | Ex8-62A>C | E429A | Biotinylated 5′-7AGGAGGAGCTGCGAAGATG (F) | Denaturing at 95°C for 6 min |
| 5′-CCCCACTCCAGCATCACTCA (R) | 45 cycles of 95°C for 15 s | ||||
| 5′-AACAAAGACTTCAAAGACAC (S) | 62°C for 30 s | ||||
| C677T | rs1801133 | Ex5+79C>T | A222V | Biotinylated 5′-7ATCCCTCGCCTTGAACAGGT (F) | 72°C for 15 s |
| 5′-TCGGTGCATGCCTTCCACAA (R) | Extension at 72°C for 6 min | ||||
| 5′-CGTGATGATGAAATCG (S) |
Abbreviations: F, forward; R, reverse; S, sequencing.