Table 1.

Descriptions for the studied SNPs and their primers and probes used for genotyping

GeneMTHFRMTHFRGSTO1GSTO1GSTO1
Chromosomal location1p36.31p36.310q25.110q25.110q25.1
Accession no.AY 338232AY 338232NM_004832NM_004832NM_004832
SNP IDrs1801133rs1801131rs4925rs11509438rs11509437
Seq 5′- to SNPagcacttgaaggagaaggtgtctgcgggagAgatgtggggsgakgagctgaccagtgaagTtattagaagccaaaataaagaagactatgaaactgaaactgtggatggcagccatgaagaatttcgtaaagaatttaccaagctagagg
Seq 3′- to SNPcgatttcatcatcacgcagcttttctttgaAagtgtctttgaagtcttygttctttacctTggcctaaaagaagaatttcgtaaagaattaagatcccacagtctcagccctgcttactataattatttctcctagctatcatcagagta
Amino acid changeAlanine 222 ValineAlanine 429 Glutamic AcidAlanine 140 Aspartic AcidGlutamic Acid 208 Lysine
Forward primer5′-tctctctgcccagtccctgt-3′5′-gagcgggagggcagaagaag-3′5′-attattctctgtctaggtgccat-3′5′-agtttctctctttgggcaagttc-3′5′-attattctctgtctaggtgccat-3′
Reverse primer5′-aagtgatgcccatgtcggtg-3′5′-ttggttctcccgagaggtaa-3′5′-aagcaagcccatgacaaagtctg-3′5′-cctcagggctgttctgtaagtag-3′5′-aagcaagcccatgacaaagtctg-3′
PCR product249 bp309 bp340 bp389 bp340 bp
Probe typeReverseForwardReverseForwardForward
Probe sequence5′-gctgcgtgatgatgaaatcg-3′5′-gggggaggagctgaccagtgaag-3′5′-gtaaattctttacgaaattcttcttttaggcca-3′5′-gaaactgtggatggcagccatgaag-3′5′-cgtaaagaatttaccaagctagagg-3′
AcycloPrime dye usedG/AC/AG/TG/AA/T
Base extension step: no. cycles2020302025