Table 4.

Error rates for Taqman, LightCycler, PCR, and RFLP

MethodGenotype error (95% confidence interval)Allele errorComments
Knowledge of 3rd allele not necessary
    Sequencing0.014 (0.000, 0.078)0.007 (0.000, 0.040)1 error, 68 correct, 4 missing
    Sequenome0.000 (0.000, 0.052)0.000 (0.000, 0.026)0 errors, 69 correct, 4 missing
    LightCycler0.096 (0.039, 0.188)0.062 (0.029, 0.114)7 errors, 66 correct, 0 missing
Knowledge of 3rd allele necessary
    Taqman0.010 (0.041, 0.195)0.050 (0.020, 0.100)7 errors, 63 correct, 3 missing
    PCR0.145 (0.072, 0.250)0.072 (0.035, 0.129)10 errors, 59 correct, 4 missing
    RFLP0.110 (0.049, 0.205)0.062 (0.029, 0.114)8 errors, 65 correct, 0 missing
  • NOTE: Genotype error rate was defined as the number of correct genotypes divided by the number of successful genotypes. Allele error rate is defined as number of correctly called alleles divided by twice the number of successful genotypes. A successful genotype is a genotype that is not missing and that is not unclear.