Table 3.

The observed genotype frequencies obtained using the different methods of analysis (see Materials and Methods)

Method (no. genotypes)Estimated allele frequency
GTA
PCR (69)0.5430.4570.000
Taqman (71)0.5860.4140.000
RFLP (73)0.5820.4180.000
LightCycler (73)0.5890.3770.034
Sequencing (69)0.5720.3840.043
Sequenom (69)0.5650.3910.043
True genotype frequency (73)0.5620.3970.041
  • NOTE: The number of genotypes is <73 for some platforms due to failed or unclear assays (see Table 4).