Table 1.

Summary of first PCR and SSP primer sets along with annealing temperatures for COMT polymorphisms in exons 3 and 4

Exon 3First PCR primerSequenceAnnealing
E3-Sgctggaacgagttcatcctg55°C
E3B-ASctgctcgcagtaggtgtcaa
Codon
Allele
SSP Primer
Sequence
Annealing
62C62-C-S with E3B-ASagcagcgcatcctgaaccac65°C
T62-T-S with E3B-ASagcagcgcatcctgaaccat
72G72-G-AS with E3-Sgcctccagcacgctctgtgc65°C
T72-T-AS with E3-Sgcctccagcacgctctgtga
Exon 4
First PCR primer
Sequence
Annealing
E4B-Scatcaccatcgagatcaacc55°C
E4-ASccctttttccaggtctgaca
Codon
Allele
SSP Primer
Sequence
Annealing
158G158-G-AS with E4B-Sgcatgcacaccttgtccttcac65°C
A158-A-AS with E4B-Sgcatgcacaccttgtccttcat
  • Abbreviation: S, sense; AS, antisense.