Table 1.

Characterization of the antibodies and the assays used

AntibodyManufacturerAntigen retrievalDilution
pRb/p105Biogenex Menarini (Italy)1 mmol/L EDTA (pH 8.0) by heating in a microwave1:200
pRb/p107DBA (Italy)1 mmol/L EDTA (pH 8.0) by heating in a pressure cooker1:500
pRb/p130Prodotti Gianni (Italy)1 mmol/L EDTA (pH 8.0) by heating in a microwave1:100
E2F4Bio-Optica (Italy)1 mmol/L EDTA (pH 8.0) by heating in a microwave1:100
Cyclin ADBA, Italy (Novocastra)1 mmol/L EDTA (pH 8.0) by heating in a microwave1:100
p21CIP1/WAF1/SDI1Inalco Division (Calbiochem)1 mmol/L EDTA (pH 8.0) by heating in a microwave1:100
Ki-67DakoCytomation (Denmark)1 mmol/L EDTA (pH 8.0) by heating in a microwave1:50