Table 1.

Dilutions, retrieval conditions, and sources of primary antibodies used for the immunohistochemical assays

AntigensM/PAntibody cloneSourceRetrieving bufferRetrieving conditions (MW)Dilution
Ki-67MMIB-1DAKO10 mmol/L Tris/HCl, 1 mmol/L EDTA (pH 9)20 min1:80
p16M16P07Neomarkers (Fremont, CA)0.5 mmol/L EDTA (pH 8)2 × 5 min1:1600
Cyclin D1MAM29Zymed (San Francisco, CA)0.5 mmol/L EDTA (pH 8)3 × 5 min1:50
Cyclin EM13A3Novocastra (Newcastle, UK)MS Unmasker (Microm, France; pH 8)3 × 5 min1:10
p53MDO-7Novocastra10 mmol/L Citrate (pH 7)20 min1:200
p21MSX118DAKO10 mmol/L Tris/HCl, 1 mmol/L EDTA (pH 9)20 min1:50
Bcl-2MC-2Santa Cruz Biotechnology, Inc. (Santa Cruz, CA)10 mmol/L Citrate (pH 6)2 × 15 min1:100
BaxMB-9Santa Cruz Biotechnology10 mmol/L Citrate (pH 6)2 × 15 min1:100
MMP-9PNeomarkers1:100
VEGFPSanta Cruz Biotechnology10 mmol/L Citrate (pH 6)2 × 15 min1:250
hnRNP A1M4B10G. Dreyfuss (Philadelphia, PA)1:6000
hnRNP KPUniversity of Navarra10 mmol/L Citrate (pH 6)2 × 15 min1:100
αCP-4PUniversity of Navarra1:50
  • Abbreviations: M, mouse monoclonal antibody; P, rabbit polyclonal antibody; MW, microwave heating.