Table 1.

Primers used in this study

PrimerPCR product (bp)Fragment sizes (bp) after BstU1 digestion
pro/proarg/argpro/arg
PCR for genomic DNA
    Sense: 5′-TCAGACACTGGCATGGTGTT-3′880880600, 280880, 600, 280
    Antisense: 5′-AAGCCTAAGGGTGAAGAGGA-3′
RT-PCR
    Sense: 5′-ATGGAGGAGCCGCAGTCAGATCCTA-3′1,200700, 500700, 280, 220700, 500, 280, 220
    Antisense: 5′-GTCTGAGTCAGGCCCTTCTGTCTTGA-3′
Primers to sequence p53 gene
    p53(1) forward: 5′-ATGGAGGAGCCGCAGTCAGATCCTA-3′
    p53(400) forward: 5′-TTGCCAACTGGCCAAGAC-3′
    p53(600) forward: 5-CTTATCCGAGTGGAAGGA-3′
    p53(800) forward: 5′-AACAGCTTTGAGGTGCGT-3′