Table 1.

MS-PCR primer sequences and reaction conditions

	Sequences	1× Buffer (and additives)	Annealing temperature (°C)
M3	S 5′-GGTTAGTAGTTCGGGTAGGGTTTATC-3′, AS 5′-CCGAATCCTACCCCGACG-3′	16.6 mmol/L (NH₄)₂SO₄	57
				67 mmol/L Tris (pH 9.1)
				3.0 mmol/L MgCl₂
U3	S 5′-TTAGTAGTTTGGGTAGGGTTTATT-3′, AS 5′-CCAAATCCTACCCCAACA-3′	15 mmol/L (NH₄)₂SO₄	57
				60 mmol/L Tris (pH 8.5)
				4.5 mmol/L MgCl₂
M4	S 5′-GTCGAGAACGCGAGCGATTC-3′, AS 5′-CGACCAATCCAACCGAAACG-3′	15 mmol/L (NH₄)₂SO₄	55
				60 mmol/L Tris (pH 9.0)
				3.5 mmol/L MgCl₂
				150 mmol/L 2-pyrrolidinone
U4	S 5′-GATGTTGAGAATGTGAGTGATTT-3′, AS 5′-AACCAATCCAACCAAAACA-3′	15 mmol/L (NH₄)₂SO₄	57
				60 mmol/L Tris (pH 8.5)
				4.5 mmol/L MgCl₂