Table 2.

STR and SNP genotyping performance of wgaDNA derived from nonirradiated and irradiated gDNA

gDNA*nNonirradiated
Irradiated
CompletedNo amp.Undet.GQ < 0.25§Concord.CompletedNo amp.Undet.GQ < 0.25§Concord.
STR gDNA (ref)24768 (100%)00768 (100%)768 (100%)01767 (100%)
    10766 (100%)20764 (100%)494 (64%)274198233 (48%)
    25768 (100%)00768 (100%)595 (78%)173134382 (69%)
    50746 (97%)2216720 (97%)497 (65%)271226258 (54%)
    100768 (100%)00768 (100%)611 (80%)157129397 (68%)
SNP gDNA (ref)242,329 (99%)0232,328 (100%)2,341 (100%)1102,341 (100%)
    102,334 (99%)1172,323 (100%)1,895 (81%)2092481,563 (82%)
    252,328 (99%)0242,271 (98%)2,018 (86%)572771,766 (88%)
    502,343 (100%)092,333 (100%)2,017 (86%)273081,855 (92%)
    1002,338 (99%)1132,328 (100%)2,060 (88%)92831,971 (96%)
  • * gDNA input (ng) into the WGA reaction.

  • No amplification observed (i.e., no STR allele observed above the threshold of 50 relative fluorescence unit, or a TaqMan data point that clusters with the no template control samples).

  • Undetermined, a TaqMan datapoint that falls outside of the three genotype clusters and the one no amplification cluster.

  • § Genotype quality scores, a composite measure of STR genotype quality.

  • Concordance compared with gDNA.

  • P < 0.0001, completion and concordance rates, wgaDNA derived from irradiated gDNA vs. nonirradiated gDNA, irradiated gDNA, and wgaDNA derived from nonirradiated gDNA.