Table 1.

wgaDNA yields (in nanograms) derived from nonirradiated and irradiated gDNA

QuantificationgDNA input (ng)Nonirradiated
Irradiated
P*
MeanSDMeanSD
A1018,4071,26818,4401,8070.94
2519,2072,40618,7832,0050.45
5018,7051,63318,6132,2540.84
10017,5592,37418,9051,9660.05
Ptrend0.040.87
Real time-PCR§103,9058911,0281,359<0.0001
254,2161,0351,3591,493<0.0001
503,9151,6101,4942,2490.0002
1004,5571,4771,3372,010<0.0001
Ptrend0.260.84
% ssDNA10473643<0.0001
25487674<0.0001
50455685<0.0001
1004412735<0.0001
Ptrend0.15<0.0001
Total DNA1014,81886713,8381,2260.003
2515,3461,59913,9761,3730.0005
5015,1431,09813,7681,4930.0005
10014,3061,63613,7471,3370.15
Ptrend0.050.94
  • * Wilcoxon signed rank test of wgaDNA yield derived from nonirradiated versus irradiated gDNA.

  • A, yield of wgaDNA assuming 50 ng dsDNA/μL/A260.

  • Ptrend, P value from ANOVA.

  • § Real time-PCR, human-specific yield of wgaDNA (8).

  • % ssDNA as estimated in Materials and Methods.

  • Total DNA, the sum of ssDNA in nanograms and dsDNA in nanograms as estimated in Materials and Methods.