Table 2.

Primer and probe sequences for the TaqMan assays

GenotypePrimer sequencesReporter sequences*Amplicon length (bp)
HFE H63DF 5′-GAT GAC CAG CTG TTC GTG TTG-3′5′-VIC-CGA CTC TCA TGA TCA TA-MGB-3′93
R 5′-CCA CAT CTG GCT TGA AAT TCT ACT G-3′5′-FAM-CGA CTC TCA TCA TCA TA-MGB-3′
HFE C282YF 5′-GGC TGG ATA ACC TTG GCT GTA C-3′5′- VIC-CCT GGC ACG TAT AT-MGB-3′75
R 5′-TCC AGG CCT GGG TGC TG-3′5′-FAM-A CCT GGT ACG TAT AT C-MGB-3′
AAT E342KF 5′-GCC TGG GAT CAG CCT TAC AAC GT-3′5′- VIC-AAC ATC GAC GAG AAA-MGB-3′
R 5′-CAT GGG TAT GGC CTC TAA AAA CAT GG-3′5′-FAM-CC ATC GAC AAG AAA-MGB-3′127
CFTR ΔF508F 5′-GAT TAT GCC TGG CAC CAT TAA AG-3′5′-VIC-A ACA CCA AAG ATG ATA TT-MGB-3′79
R 5′-TGC TTT GAT GAC GCT TCT GTA TCT A-3′5′-FAM-CA CCA ATG ATA TTT T-MGB-3′76
  • NOTE: F, forward primer; R, reverse primer.

  • * Genetic variants are in boldface.