Table 1.

Primer sequences for the gel electrophoresis assays

GenotypePrimer sequencesAmplicon length (bp)
HFE H63DForward 5′-GAC CTT GGT CTT TCC TTG TTT GAA GC-3′90
Reverse 5′-GGG CTC CAC ACG GCG AC-3′
HFE C282YForward 5′CCA GGG CTG GAT AAC CTT GGC T-3′101
Reverse 5′-CCC AGA TCA CAA TGA GGG GCT G-3′
AAT E342KForward 5′-GCC TGG GAT CAG CCT TAC AAC GT-3′127
Reverse 5′-CAT GGG TAT GGC CTC TAA AAA CAT GG-3′
PiZ ASO + 5′-CAT AAG GCT GTG CTG ACC ATC AAC AA-3′PiZ, 75
PiM ASO − 5′-CCA GCA GCT TCA GTC CCT TCC TC-3′PiM, 99
CFTR ΔF508Forward 5′-TTC TGT TCT CAG TTT TCC TGG AT-3′120
Reverse 5′-TCT TAC CTC TTC TAG TTG GCA TG-3′
Inner F 5′-GCA CCA TTA AAG AAA ATA TCA TCT T-3′89
Inner R 5′-ACC AAT GAT ATC TTC TTT AAT GGT G-3′57