Table 1.

Primer sequences, condition for amplification, restriction pattern, and restriction enzymes used

PolymorphismsPrimer sequencesAnnealing temperature (°C)Length (bp)Restriction enzymeRestriction pattern (bp)
XRCC1 Arg194TrpF: 5′-GCCCCGTCCCAGGTA-3′58491MspIArg/Arg: 292, 178, 21; Arg/Trp: 313, 292, 178, 21; Trp/Trp: 313, 178
R: 5′-AGCCCCAAGACCCTTTCACT-3′
XRCC1 Arg280HisF: 5′-TGGGGCCTGGATTGCTGGGTCTG-3′69.5280RsaIArg/Arg: 280; Arg/His: 280, 140; His/His: 140
R: 5′-CAGCACCACTACCACACCCTGAAGG-3′
XRCC1 Arg399GlnF: 5′-TTGTGCTTTCTCTGTGTCCA-3′56615MspIArg/Arg: 377, 238; Arg/Gln: 615, 377, 238; Gln/Gln: 615
R: 5′-TCCTCCAGCCTTTTCTGATA-3′
APE1 Asp148GluF: 5′-CTGTTTCATTTCTATAGGCTA-3′*54165BfaIGlu/Glu: 146, 19; Glu/Asp: 165, 146, 19; Asp/Asp: 165
R: 5′-AGGAACTTGCGAAAGGCTTC-3′
ADPRT Val762AlaF: 5′-TTTTGCTCCTCCAGGCCAACG-3′*57156Bsh1236IVal/Val: 156; Val/Ala: 156, 135, 21; Ala/Ala: 135, 21;
R: 5′-CCTGACCCTGTTACCTTAATGTCAGTTTT-3′
XRCC2 Arg188HisF: 5′-GCGTCAATGGAGGAGAAAGTGTGAA-3′55113PmacIArg/Arg: 113; Arg/His: 113, 89, 24; His/His: 89, 24
R: 5′-TTGTGTCGTTGCAAAAAAACACG-3′*
XRCC3 Thr241MetF: 5′-GGTCGAGTGACAGTCCAAAC-3′60456NlaIIIThr/Thr: 316, 140; Thr/Met: 316, 211, 140, 105; Met/Met: 211, 140, 105
R: 5′-TGCAACGGCTGAGGGTCTT-3′
  • * Underlined base modifies primer sequence, introducing a restriction site in the presence of the A or C nucleotide.