Table 1.

Genotyping assay conditions

PolymorphismPrimers and probes[MgCl], mmol/LCycling
MGMT p.L84F
    FP5′-CCCGAGGCTATCGAAGAGTTC-3′495°C, 15 s; 60°C, 60 s
    RP5′-GTTACCGACCTTGCTGGAAAAC-3′
    L845′VIC-CCGGCTCTTCACCA-NFQ3′
    F845′6FAM-CCGGCTTTTCACC-NFQ3′
MGMT p.I143V
    FP5′-TTGACCCCAAAGACCTCGTT-3′395°C, 15 s; 60°C, 60 s
    RP5′-CGCTGCTGCAGACCACTCT-3′
    I1435′VIC-CCATCCTCATCCCGT-NFQ3′
    V1435′6FAM-CCATCCTCGTCCCGT-NFQ3′
XPD p.D312N
    FP5′-AGCTCATCTCTCCGCAGGATCAA-3′Universal master mix94°C, 30 s; 64°C, 45 s; 72°C, 60 s
    RP5′-CCTCTGCGAGGAGACGCTATCAG-3′
    D3125′VIC-CTTCGTCGGGCAGCA-NFQ3′
    N3125′6FAM-CTTCGTTGGGCAGCAC-NFQ3′
XPD p.K751Q
    FP5′-CCCTCTCCCTTTCCTCTGTTCT-3′395°C, 15 s; 60°C, 60 s
    RP5′-CACTCAGAGCTGCTGAGCAATC-3′
    K7515′VIC-ATCCTCTTCAGCGTCT-NFQ3′
    Q7515′6FAM-TCCTCTGCAGCGTC-NFQ3′
XPG p.D1104H
    FP5′-ATACATGCGGTGGATTTTTGG-3′495°C, 15 s; 60°C, 60 s
    RP5′-CGCAGCTGTTCTCCTTTGTACA-3′
    D11045′VIC-TCAAGTGAAGATGCTGAA-NFQ3′
    H11045′6FAM-CAAGTGAACATGCTGAA-NFQ3′