Table 1.

Stability of whole blood samples on ice from 0 to 36 hours before processing (n = 6)

Median (Interquartile Range)
P for Paired t Test
ICC
0 Hours24 Hours36 Hours0-24 Hours0-36 Hours0-24 Hours0-36 Hours
Analysis based on single measurement per subject
MDA (μmol/L) via TBA assay1.94 (1.59-2.50)2.92 (2.87-3.33)3.85 (3.03-4.70)0.030.001−0.09−0.11
Esterified F2-isoprostanes* (pg/mL)46.0 (35.0-69.0)83.0 (76.0-95.0)129.5 (84.0-183.0)0.050.010.450.09
Fluorescent assay (fluorescent intensity/mL)164 (127-190)163 (134-200)163 (134-194)0.30.50.960.98
Analysis based on duplicate measures per subject
MDA (μmol/L) via TBA assay1.97 (1.53-2.43)3.21 (2.91-3.54)3.71 (3.32-4.6)0.010.0030.160.02
Esterified F2-isoprostanes (pg/mL)55.3 (47.5-76.5)73.5 (69.0-88.5)101.3 (68.3-123.5)0.040.020.740.51
Fluorescent assay (fluorescent intensity/mL)177 (129-208)170 (145-202)166 (142-198)0.70.30.970.97
  • * Data were log transformed.

  • Geometric means are presented.