Table 1.

Summary of methods for the five polymorphisms evaluated in the study

Locations*
Sequence changePCR primersAnnealing temperature (°C)EnzymeAllelesRestriction fragments (bp)
AB
−2021,704ACF: 5′-GAATGCGGAGCGCTGTATG-3′59FspIA483
R: 5′-TGTGGAATCCAGGCAGGAAG-3′C258,225
2272,132GC (G32A)F: 5′-GCCTGGATTCCACAGCTTC-3′55AvaIG271,179
R: 5′-TGACGGCACTAGCGTTGAC-3′C450
3,8045,709CGF: 5′-ACTGAAGCGTTTGTCTGCCT-3′52BsmAIC264,98,77
R: 5′-AACTTTGTAGCGCTGGCTGT-3′G341,98
5,6067511Insertion AF: 5′-CTCCGAGTCACTGGCATTTC-3′55NdeINo insertion A611
R: 5′-ACCAGCCCTTGTAGAACCTC-3′Insertion A443,168
5,8277,732CTIdentical to those for 7,511 insertion A55BtsIC397,214
T661
  • * A, location relative to the transcription start site described by Cubbage et al. (21); B, location based on the sequence from Genbank accession no. M35878.