Table 2.

Sequences of primers and masses of extension products of MALDI-TOF MS assays

Single nucleotide polymorphismPrimerSequenceMass (kDa)
ERCC2_6540_G>APCR primer 15′-ACGTTGGATGTGCGAGGAGACGCTATCAGC-3′
PCR primer 25′-ACGTTGGATGAGTACCGGCGTCTGGTGGAG-3′
Extension primer5′-CTCACCCTGCAGCACTTCGT-3′5,988.9
Analyte G5′-CTCACCCTGCAGCACTTCGTC-3′6,262.0
Analyte A5′-CTCACCCTGCAGCACTTCGTTG-3′6,606.0
ERCC2_18880_A>CPCR primer 15′-ACGTTGGATGAGCAGCTAGAATCAGAGGAG3′
PCR primer 25′-ACGTTGGATGCACCAGGAACCGTTTATGGC-3′
Extension primer5′-GAGCAATCTGCTCTATCCTCT-3′6,332.1
Analyte A5′-GAGCAATCTGCTCTATCCTCTT-3′6,620.3
Analyte C5′-GAGCAATCTGCTCTATCCTCTGC-3′6,934.5