Table 1

Design of primers and fluorogenic probes for LightCycler

RAI intron 1
 Forward primer: 5′-TGGCTAACACGGTGAAACC
 Reverse primer: 5′-GGAATCCAAAGATTCTATGATGG
 Anchor probe: 5′-GGGAGGCGGAGCTTGCAGTGA-fluorescein
 Sensor probe: 5′-LCRed 640-CTGAGATCGCACCACTGCAC-p
CKM exon 8
 Forward primer: 5′-TTGAAACTGGAACTCTGAGAAGG
 Reverse primer: 5′-TGGTGGATGGTGTGAAGCA
 Anchor probe: 5′-LCRed 640-CCTTTCTCCAACTTCTTCTCCATTTCCACC-p
 Sensor probe: 5′-GGGGATCATGTCGTCAATGGACT-fluorescein
ERCC1 exon 4
 Forward primer: 5′-AGGACCACAGGACACGCAGA-3′
 Reverse primer: 5′-CATAGAACAGTCCAGAACAC-3′
 Anchor probe: 5′-LCRed640-TGGCGACGTAATTCCCGACTATGTGCTG p-3′
 Sensor probe: 5′-CGCAACGTGCCCTGGGAAT-fluorescein
FOSB exon 4
 Forward primer: 5′-AGGCTCAACAAGGAAAAATGC
 Reverse primer: 5′-GCTAGACAGTCAAGGAGGGACG
 Anchor probe: 5′-LCRed 640-AAAGGGTGGGTGTGGGAGACATTGG-p
 Sensor probe: 5′-AAACCAACCTAGGCACCCCAAA-fluorescein
SLC1A5 exon 8
 Forward primer: 5′-CAGTGTCCAAAGAGCACC
 Reverse primer: 5′-CTACCCCTTTAGCGACC
 Anchor probe: 5′-LCRed 640-TCCTGCCCCCAGAGCGTCACC-p
 Sensor probe: 5′-GTACGGTCCACATAATTTTGGAGGA-fluorescein
LIG1 exon 6
 Forward primer: 5′-ATGCCCTGTAGGTTCAATGG
 Reverse primer: 5′-TGGAGGTCTTTAGGGGCTTG
 Anchor probe: 5′-GGCTGGTCCCCGTCTTCTCCTTCC-fluorescein
 Sensor probe: 5′-LCRed 640-TCTCTGTTGCCACTTCAGCCTC-p
GLTSCR1 exon 1
 Forward primer: 5′-CGACGAACTTCTCTGAAGCGAA
 Reverse primer: 5′-AGCGACACGGGCATCTGG
 Anchor probe: 5′-ATGAGCGTCCACCTCCTGAACC-fluorescein
 Sensor probe: 5′-LCRed 640-AGGCAGCAGCATCGTCATCCCC-p