Table 1

Amplification and detection parameters for ERCC2 polymorphism detection

Primer namePrimer sequenceAnnealing temperatureDetection (polymorphism, gene location)a
XPD6F5′ TGG AGT GCT ATG GCA CGA TCT CT 3′55°CTfi I digestion (R156R, C to A, exon 6)
XPD6R5′ CCA TGG GCA TCA AAT TCC TGG GA 3′
XPD811F5′ GGC CTG TGT GGG AGT GAC GG 3′59°CBstYI digestion (I199M, C to G, exon 8), Sphl digestion (H201Y, C to T, exon 8)
N-5′ GTG CTG CCC GAC GAA GT 3′b
M-5′ ACT TCG TTG GGC AGC AC 3′b (D312N, G to A, exon 10)
XPD811R5′ GGA CAC GGC TCT GCA TAA CC 3′
XPD1819F5′ CTG TGC CCT GAA CCC ACC CTG 3′55°CBanI digestion (A575A, C to T, exon 18)
XPD1819R5′ AGA GAG CTC TGG GAA GAC ACC 3′
XPD2021F5′ CCC CAA CTC AGA CAC AGC AT 3′57°CBsaHI digestion (R616C, C to T, exon 20)
XPD2021R5′ CGC ATG GCA TCG AAG GTA AG 3′
XPD2122F5′ GGC TGT TTC CCG TTC ATT TC 3′59°CN-5′ ACC GTG GAC GAG GGT GT 3′b
M-5′ ACA CCC TCA TCC ACG GT 3′b (D711D, C to T, exon 22)
XPD2122R5′ GTA GAT GCA CGA TAA ACT TC 3′
XPD23F5′ CCC CCT CTC CCT TTC CTC TG 3′59°CMboII digestion (K751Q, A to C, exon 23)
XPD23R5′ AAC CAG GGC CAG GCA AGA C3′
  • a Bold, mutation names.

  • b Detection via allele-specific oligonucleotide hybridization.