RT Journal Article
SR Electronic
T1 Improved Stool DNA Integrity Method for Early Colorectal Cancer Diagnosis
JF Cancer Epidemiology Biomarkers & Prevention
JO Cancer Epidemiol Biomarkers Prev
FD American Association for Cancer Research
SP 2553
OP 2560
DO 10.1158/1055-9965.EPI-14-0379
VO 23
IS 11
A1 Rengucci, Claudia
A1 De Maio, Giulia
A1 Menghi, Maura
A1 Scarpi, Emanuela
A1 Guglielmo, Simona
A1 Fusaroli, Pietro
A1 Caletti, Giancarlo
A1 Saragoni, Luca
A1 Gardini, Andrea Casadei
A1 Zoli, Wainer
A1 Falcini, Fabio
A1 Amadori, Dino
A1 Calistri, Daniele
YR 2014
UL http://cebp.aacrjournals.org/content/23/11/2553.abstract
AB Background: DNA integrity analysis could represent an alternative approach to the early detection of colorectal cancer. Previously, fluorescence long DNA (FL-DNA) in stools was extracted using a manual approach and analyzed by capillary electrophoresis assay (CE FL-DNA). We aimed to improve diagnostic accuracy using a simpler and more standardized method [Real Time PCR FL-DNA (RT FL-DNA)] for the detection of early malignant lesions in a population undergoing colorectal cancer screening. Methods: From 241 stool samples, DNA was extracted using manual and semiautomatic extraction systems and analyzed using FL-DNA tests by CE and RT assays. The RT FL-DNA approach showed slightly higher sensitivity and specificity compared with the CE FL-DNA method. Furthermore, we compared the RT FL-DNA approach with the iFOBT report. Results: Nonparametric ranking statistics were used to analyze the relationship between the median values of RT FL-DNA and the clinicohistopathologic characteristics. The median values of both variables were significantly higher in patients with cancer than in patients with noncancerous lesions. According to the Fagan nomogram results, the iFOBT and FL-DNA methods provided more accurate diagnostic information and were able to identify subgroups at varying risks of cancer. Conclusions: The combination of the semiautomatic extraction system and RT FL-DNA analysis improved the quality of DNA extracted from stool samples. Impact: RT FL-DNA shows great potential for colorectal cancer diagnosis as it is a reliable and relatively easy analysis to perform on routinely processed stool samples in combination with iFOBT. Cancer Epidemiol Biomarkers Prev; 23(11); 2553–60. ©2014 AACR.