PT  - JOURNAL ARTICLE
AU  - Chaturvedi, Anil K.
AU  - Kemp, Troy J.
AU  - Pfeiffer, Ruth M.
AU  - Biancotto, Angelique
AU  - Williams, Marcus
AU  - Munuo, Stella
AU  - Purdue, Mark P.
AU  - Hsing, Ann W.
AU  - Pinto, Ligia
AU  - McCoy, J. Philip
AU  - Hildesheim, Allan
TI  - Evaluation of Multiplexed Cytokine and Inflammation Marker Measurements: a Methodologic Study
AID  - 10.1158/1055-9965.EPI-11-0221
DP  - 2011 Sep 01
TA  - Cancer Epidemiology Biomarkers & Prevention
PG  - 1902--1911
VI  - 20
IP  - 9
4099  - http://cebp.aacrjournals.org/content/20/9/1902.short
4100  - http://cebp.aacrjournals.org/content/20/9/1902.full
SO  - Cancer Epidemiol Biomarkers Prev2011 Sep 01; 20
AB  - Background: Chronic inflammation is etiologically related to several cancers. We evaluated the performance [ability to detect concentrations above the assay's lower limit of detection, coefficients of variation (CV), and intraclass correlation coefficients (ICC)] of 116 inflammation, immune, and metabolic markers across two Luminex bead–based commercial kits and three specimen types. Methods: From 100 cancer-free participants in the Prostate, Lung, Colorectal, and Ovarian Cancer Trial, serum, heparin plasma, and EDTA plasma samples were utilized. We measured levels of 67 and 97 markers using Bio-Rad and Millipore kits, respectively. Reproducibility was assessed using 40 blinded duplicates (20 within-batches and 20 across-batches) for each specimen type. Results: A majority of markers were detectable in more than 25% of individuals on all specimen types/kits. Of the 67 Bio-Rad markers, 51, 52, and 47 markers in serum, heparin plasma, and EDTA plasma, respectively, had across-batch CVs of less than 20%. Likewise, of 97 Millipore markers, 75, 69, and 78 markers in serum, heparin plasma, and EDTA plasma, respectively, had across-batch CVs of less than 20%. When results were combined across specimen types, 45 Bio-Rad and 71 Millipore markers had acceptable performance (>25% detectability on all three specimen types and across-batch CVs <20% on at least two of three specimen types). Median concentrations and ICCs differed to a small extent across specimen types and to a large extent between Bio-Rad and Millipore. Conclusions: Inflammation and immune markers can be measured reliably in serum and plasma samples using multiplexed Luminex-based methods. Impact: Multiplexed assays can be utilized for epidemiologic investigations into the role of inflammation in cancer etiology. Cancer Epidemiol Biomarkers Prev; 20(9); 1902–11. ©2011 AACR.