PT  - JOURNAL ARTICLE
AU  - Kolwijck, Eva
AU  - Zusterzeel, Petra L.M.
AU  - Roelofs, Hennie M.J.
AU  - Hendriks, Jan C.
AU  - Peters, Wilbert H.M.
AU  - Massuger, Leon F.A.G.
TI  - GSTP1-1 in Ovarian Cyst Fluid and Disease Outcome of Patients With Ovarian Cancer
AID  - 10.1158/1055-9965.EPI-09-0098
DP  - 2009 Aug 01
TA  - Cancer Epidemiology Biomarkers & Prevention
PG  - 2176--2181
VI  - 18
IP  - 8
4099  - http://cebp.aacrjournals.org/content/18/8/2176.short
4100  - http://cebp.aacrjournals.org/content/18/8/2176.full
SO  - Cancer Epidemiol Biomarkers Prev2009 Aug 01; 18
AB  - Detoxification enzymes, especially glutathione S-transferase P1-1 (GSTP1-1), have been implicated in resistance to platinum-based chemotherapy. We studied GSTP1-1 levels in ovarian cyst fluid (oCF), obtained during surgery before chemotherapy, of patients with epithelial ovarian cancer and clinical outcomes were correlated. GSTP1-1 was determined by ELISA in oCF of 56 patients with epithelial ovarian cancer and 109 noncancer controls (21 borderline and 88 benign ovarian tumors). Differences in median GSTP1-1 between clinicopathologic subgroups were studied using Mann-Whitney U and Kruskal Wallis tests. Differences in disease-free (DFS) and overall survival (OS) between groups were analyzed by applying Kaplan-Meyer estimates and log-rank tests. Univariate and multivariate analysis were done using Cox proportional hazard model. Significantly higher levels of GSTP1-1 were found in the oCF of malignant (median, 383; range, 10-32,695 ng/mL) compared with benign (median, 20; range, 0-1,128 ng/mL) ovarian tumors (P < 0.01). Significantly higher GSTP1-1 levels were found in patients with advanced International Federation of Gynaecologists and Obstetricians stage (P = 0.01), high-grade tumors (P = 0.44), and/or high levels of preoperative CA 125 (P = 0.01). Of patients who received chemotherapy (stage, ≥Ic; n = 30), high GSTP1-1 levels were significantly associated with a poor DFS and OS (log-rank P = 0.047 and P = 0.033, respectively). International Federation of Gynaecologists and Obstetricians stage was the only independent predictor for DFS. GSTP1-1 was the only independent predictor for OS. (Cancer Epidemiol Biomarkers Prev 2009;18(8):2176–81)