PT - JOURNAL ARTICLE AU - Kolwijck, Eva AU - Zusterzeel, Petra L.M. AU - Roelofs, Hennie M.J. AU - Hendriks, Jan C. AU - Peters, Wilbert H.M. AU - Massuger, Leon F.A.G. TI - GSTP1-1 in Ovarian Cyst Fluid and Disease Outcome of Patients With Ovarian Cancer AID - 10.1158/1055-9965.EPI-09-0098 DP - 2009 Aug 01 TA - Cancer Epidemiology Biomarkers & Prevention PG - 2176--2181 VI - 18 IP - 8 4099 - http://cebp.aacrjournals.org/content/18/8/2176.short 4100 - http://cebp.aacrjournals.org/content/18/8/2176.full SO - Cancer Epidemiol Biomarkers Prev2009 Aug 01; 18 AB - Detoxification enzymes, especially glutathione S-transferase P1-1 (GSTP1-1), have been implicated in resistance to platinum-based chemotherapy. We studied GSTP1-1 levels in ovarian cyst fluid (oCF), obtained during surgery before chemotherapy, of patients with epithelial ovarian cancer and clinical outcomes were correlated. GSTP1-1 was determined by ELISA in oCF of 56 patients with epithelial ovarian cancer and 109 noncancer controls (21 borderline and 88 benign ovarian tumors). Differences in median GSTP1-1 between clinicopathologic subgroups were studied using Mann-Whitney U and Kruskal Wallis tests. Differences in disease-free (DFS) and overall survival (OS) between groups were analyzed by applying Kaplan-Meyer estimates and log-rank tests. Univariate and multivariate analysis were done using Cox proportional hazard model. Significantly higher levels of GSTP1-1 were found in the oCF of malignant (median, 383; range, 10-32,695 ng/mL) compared with benign (median, 20; range, 0-1,128 ng/mL) ovarian tumors (P < 0.01). Significantly higher GSTP1-1 levels were found in patients with advanced International Federation of Gynaecologists and Obstetricians stage (P = 0.01), high-grade tumors (P = 0.44), and/or high levels of preoperative CA 125 (P = 0.01). Of patients who received chemotherapy (stage, ≥Ic; n = 30), high GSTP1-1 levels were significantly associated with a poor DFS and OS (log-rank P = 0.047 and P = 0.033, respectively). International Federation of Gynaecologists and Obstetricians stage was the only independent predictor for DFS. GSTP1-1 was the only independent predictor for OS. (Cancer Epidemiol Biomarkers Prev 2009;18(8):2176–81)