RT Journal Article
SR Electronic
T1 Evidence for Selective Expression of the p53 Codon 72 Polymorphs: Implications in Cancer Development
JF Cancer Epidemiology Biomarkers & Prevention
JO Cancer Epidemiol Biomarkers Prev
FD American Association for Cancer Research
SP 2245
OP 2252
DO 10.1158/1055-9965.EPI-05-0153
VO 14
IS 9
A1 Siddique, Mobin M.
A1 Balram, Chowbay
A1 Fiszer-Maliszewska, Lucja
A1 Aggarwal, Amit
A1 Tan, Angie
A1 Tan, Patrick
A1 Soo, Khee C.
A1 Sabapathy, Kanaga
YR 2005
UL http://cebp.aacrjournals.org/content/14/9/2245.abstract
AB Polymorphism at codon 72 of p53 results in either the arginine or proline form of p53, whose functional significance in carcinogenesis is controversial. We have investigated if the expression of these p53 polymorphs is selectively regulated, using mRNA from peripheral blood of healthy Asian (Chinese) and the Caucasian (Polish) arginine/proline (arg/pro) heterozygote subjects. Asians were found to preferentially express the pro allele whereas the Caucasians preferentially express the arg allele. On the contrary, about 75% of the heterozygote Chinese breast cancer patients preferentially expressed the arg allele, which rarely contained any somatic mutations. Moreover, histologically normal tissues from Chinese heterozygote breast cancer patients showed selective expression of the arg allele, in contrast to the preferential expression of the pro allele in heterozygote healthy normal breast tissues. Together, the data suggest that the expression of the different p53 polymorphs is selectively regulated in different ethnic populations, and that the arg allele is activated during cancer development in Asians. Thus, the expression status of the p53 polymorphs, rather than the genotypic status, might be a useful indicator for cancer susceptibility.