PT  - JOURNAL ARTICLE
AU  - Dong, Seung Myung
AU  - Pai, Sara I.
AU  - Rha, Seo-Hee
AU  - Hildesheim, Allan
AU  - Kurman, Robert J.
AU  - Schwartz, Peter E.
AU  - Mortel, Rodrigue
AU  - McGowan, Larry
AU  - Greenberg, Mitchell D.
AU  - Barnes, Willard A.
AU  - Sidransky, David
TI  - Detection and Quantitation of Human Papillomavirus DNA in the Plasma of Patients with Cervical Carcinoma
DP  - 2002 Jan 01
TA  - Cancer Epidemiology Biomarkers & Prevention
PG  - 3--6
VI  - 11
IP  - 1
4099  - http://cebp.aacrjournals.org/content/11/1/3.short
4100  - http://cebp.aacrjournals.org/content/11/1/3.full
SO  - Cancer Epidemiol Biomarkers Prev2002 Jan 01; 11
AB  - Human papillomaviruses (HPVs) play a central role in the development of cervical carcinoma. Plasma DNA from 232 patients taken at diagnosis or after treatment for invasive cervical cancer (n = 175) or carcinoma in situ (n = 57) and 60 normal controls were examined for HPV-16 or HPV-18 E7 DNA by conventional and real-time quantitative PCR assays. We found HPV-16 or HPV-18 E7 DNA in 6.9% (11 of 175) of invasive cervical cancer cases (18.1% of cases positive for HPV-16 or HPV-18 at the genital tract), 1.8% (1 of 57) of carcinoma in situ, and 1.7% (1 of 60) of normal controls by conventional PCR. Quantitative PCR identified the highest concentrations of HPV DNA (copy number of HPV/ml of plasma) in patients with invasive cervical cancer (mean, 11,163; median, 183.5), followed by a level of 8 in the single carcinoma in situ case and 0 copies in the normal control initially positive by conventional PCR. HPV DNA can be detected in the plasma of some patients with HPV-positive cervical tumors. It remains to be demonstrated whether quantitative PCR analysis of HPV DNA in plasma may have utility in patients at high risk of recurrent disease.