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Cancer Epidemiology, Biomarkers & Prevention
Cancer Epidemiology, Biomarkers & Prevention
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Research Articles

Discovery and Validation of a Serologic Autoantibody Panel for Early Diagnosis of Esophageal Squamous Cell Carcinoma

Jianbo Pan, Qing-Zhu Zheng, Yadong Li, Li-Li Yu, Qing-Wei Wu, Jia-Ying Zheng, Xiao-Jie Pan, Bao-Song Xie, Yan-An Wu, Jiang Qian, Heng Zhu and Yi Huang
Jianbo Pan
1Department of Ophthalmology, Johns Hopkins School of Medicine, Baltimore, Maryland.
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Qing-Zhu Zheng
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
3Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, China.
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Yadong Li
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
3Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, China.
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Li-Li Yu
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
3Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, China.
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Qing-Wei Wu
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
3Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, China.
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Jia-Ying Zheng
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
3Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, China.
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Xiao-Jie Pan
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
4Department of Thoracic Surgery, Fujian Provincial Hospital, Fuzhou, China.
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Bao-Song Xie
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
5Department of Respiratory Medicine, Fujian Provincial Hospital, Fuzhou, China.
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Yan-An Wu
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
3Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, China.
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Jiang Qian
1Department of Ophthalmology, Johns Hopkins School of Medicine, Baltimore, Maryland.
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  • For correspondence: Hyi8070@126.com Hzhu4@jhmi.edu Jiang.qian@jhmi.edu
Heng Zhu
6Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland.
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  • For correspondence: Hyi8070@126.com Hzhu4@jhmi.edu Jiang.qian@jhmi.edu
Yi Huang
2Provincial Clinical College, Fujian Medical University, Fuzhou, China.
3Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou, China.
7Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou, China.
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  • For correspondence: Hyi8070@126.com Hzhu4@jhmi.edu Jiang.qian@jhmi.edu
DOI: 10.1158/1055-9965.EPI-18-1269 Published September 2019
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Abstract

Background: Esophageal squamous cell carcinoma (ESCC) accounts for the highest incidence rate worldwide and is responsible for the fourth leading cause of cancer-related death. Currently, serologic biomarkers for early ESCC diagnosis are needed for timely treatment.

Methods: The performance of a four-autoantibody panel (i.e., anti-TP53, HRAS, CTAG1A, and NSG1) was evaluated by ELISA for the early diagnosis of ESCC with 569 retrospective serum samples. A training set comprising 129 patients with early-stage ESCC, 130 patients with esophageal benign lesion (EBL), and 150 healthy controls (HC) was used to develop an early ESCC predictive model. Data obtained from an independent validation set were used to evaluate and validate the predictive model to distinguish the early ESCC from the controls (EBL+HC). Finally, a multiplexed assay based on the Luminex xMAP technology platform was developed to enable simultaneous detection of the four-autoantibody panel using the validation set.

Results: The four-autoantibody panel significantly discriminated early ESCC cases from the controls with 62.8% sensitivity at 88.9% specificity in the training set and with 58.0% sensitivity at 90.0% specificity in the independent validation set. The results of the multiplexed assay using xMAP technology for early ESCC showed a significant correlation with that of the ELISA assays with 66.0% sensitivity at 90.9% specificity.

Conclusions: A four-autoantibody panel showed good performance for early ESCC diagnosis with ELISA and could be further developed into a multiplex assay using the Luminex xMAP technology.

Impact: The four-autoantibody panel could be used for serologic screening for early ESCC.

Footnotes

  • Note: Supplementary data for this article are available at Cancer Epidemiology, Biomarkers & Prevention Online (http://cebp.aacrjournals.org/).

  • Cancer Epidemiol Biomarkers Prev 2019;28:1454–60

  • Received November 30, 2018.
  • Revision received February 14, 2019.
  • Accepted June 21, 2019.
  • Published first June 25, 2019.
  • ©2019 American Association for Cancer Research.
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Cancer Epidemiology Biomarkers & Prevention: 28 (9)
September 2019
Volume 28, Issue 9
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Discovery and Validation of a Serologic Autoantibody Panel for Early Diagnosis of Esophageal Squamous Cell Carcinoma
Jianbo Pan, Qing-Zhu Zheng, Yadong Li, Li-Li Yu, Qing-Wei Wu, Jia-Ying Zheng, Xiao-Jie Pan, Bao-Song Xie, Yan-An Wu, Jiang Qian, Heng Zhu and Yi Huang
Cancer Epidemiol Biomarkers Prev September 1 2019 (28) (9) 1454-1460; DOI: 10.1158/1055-9965.EPI-18-1269

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Discovery and Validation of a Serologic Autoantibody Panel for Early Diagnosis of Esophageal Squamous Cell Carcinoma
Jianbo Pan, Qing-Zhu Zheng, Yadong Li, Li-Li Yu, Qing-Wei Wu, Jia-Ying Zheng, Xiao-Jie Pan, Bao-Song Xie, Yan-An Wu, Jiang Qian, Heng Zhu and Yi Huang
Cancer Epidemiol Biomarkers Prev September 1 2019 (28) (9) 1454-1460; DOI: 10.1158/1055-9965.EPI-18-1269
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