Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • CEBP Focus Archive
    • Meeting Abstracts
    • Progress and Priorities
    • Collections
      • COVID-19 & Cancer Resource Center
      • Disparities Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Informing Public Health Policy
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Epidemiology, Biomarkers & Prevention
Cancer Epidemiology, Biomarkers & Prevention
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • CEBP Focus Archive
    • Meeting Abstracts
    • Progress and Priorities
    • Collections
      • COVID-19 & Cancer Resource Center
      • Disparities Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Informing Public Health Policy
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Research Articles

Postmenopausal Hormone Therapy and Breast Cancer Prognostic Characteristics: A Linkage between Nationwide Registries

Marta Román, Sidsel Graff-Iversen, Elisabete Weiderpass, Siri Vangen, Solveig Sakshaug, Solveig Hofvind and Giske Ursin
Marta Román
1Cancer Registry of Norway, Institute of Population-Based Cancer Research, Oslo, Norway.
2Women and Children's Division, National Advisory Unit for Women's Health, Oslo University Hospital, Oslo, Norway.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sidsel Graff-Iversen
3Department of Chronic Diseases, Norwegian Institute of Public Health, Oslo, Norway.
4Department of Community Medicine, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, Tromsø, Norway.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elisabete Weiderpass
1Cancer Registry of Norway, Institute of Population-Based Cancer Research, Oslo, Norway.
4Department of Community Medicine, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, Tromsø, Norway.
5Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
6Department of Genetic Epidemiology, Folkhälsan Research Center, Helsinki, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Siri Vangen
2Women and Children's Division, National Advisory Unit for Women's Health, Oslo University Hospital, Oslo, Norway.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Solveig Sakshaug
7Department of Pharmacoepidemiology, Norwegian Institute of Public Health, Oslo, Norway.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Solveig Hofvind
1Cancer Registry of Norway, Institute of Population-Based Cancer Research, Oslo, Norway.
8Oslo and Akershus University College of Applied Sciences, Faculty of Health Science, Oslo, Norway.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Giske Ursin
1Cancer Registry of Norway, Institute of Population-Based Cancer Research, Oslo, Norway.
9Department of Preventive Medicine, University of Southern California, California.
10Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Giske.Ursin@kreftregisteret.no
DOI: 10.1158/1055-9965.EPI-16-0240 Published November 2016
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Background: The effects of use of different types of hormone therapy on breast cancer risk according to prognostic factors are largely unknown.

Methods: We linked data from the Norwegian Prescription Database and the Cancer Registry of Norway during 2004 to 2009 on all women ages 45 to 79 years (N = 686,614). We estimated rate ratios and 95% confidence intervals for breast cancer in relation to hormone therapy using Poisson regression.

Results: During an average 4.8 years of follow-up, 7,910 invasive breast cancers were diagnosed. Compared with nonusers of hormone therapy, users of estradiol and tibolone were more likely to be diagnosed with grade I, lymph node–negative, and estrogen receptor–positive (ER+)/progesterone receptor–positive (PR+) tumors. However, compared with nonusers, users of the most common estrogen and progestin combinations [estradiol–norethisterone acetate (NETA) preparations (Kliogest, Activelle or Trisekvens)] were at a 4- to 5-fold elevated risk of grade I tumors, 3-fold elevated risk of lymph node–negative tumors, and 3- to 4-fold elevated risk of ER+/PR+ tumors. Importantly, estradiol–NETA users were also at a 2- to 3-fold increased risk of medium differentiated (grade II) tumors and tumors with lymph node involvement.

Conclusions: Use of oral estradiol, tibolone, and estradiol–NETA predominantly increases the risk of breast cancer with favorable prognosis characteristics. However, use of estradiol–NETA preparations also increases the risk of breast cancers with less favorable characteristics.

Impact: The hormone therapy preparations most commonly used in the Nordic countries are associated with both breast cancers with good and less favorable prognosis characteristics. Cancer Epidemiol Biomarkers Prev; 25(11); 1464–73. ©2016 AACR.

This article is featured in Highlights of This Issue, p. 1447

Introduction

There is convincing evidence that hormone therapy use is a risk factor for breast cancer (1–14). Use of combined estrogen–progestin therapy (EPT) has a substantially greater risk of breast cancer than preparations containing estrogen alone (ET; refs. 6, 7, 13–18), whereas the effect of tibolone has been less investigated (10, 19, 20).

A number of studies have reported an association between hormone therapy use and the occurrence of well-differentiated tumors with good prognostic characteristics (2, 21, 22), mostly estrogen receptor–positive (ER+) and progesterone receptor–positive (PR+) tumors (17, 23–25). Both ET and EPT have been associated with greater risks of invasive lobular cancer and tubular cancers than with invasive ductal cancer in a number of studies (21, 22, 26–33). However, there is limited evidence as to whether prognostic characteristics of tumors differ between users and nonusers of ET and EPT (17, 27, 32, 34–36). Until now, most studies have been limited by lack of statistical power or detailed information on hormone therapy types to allow for specific subgroup analyses.

We used information from the Norwegian population-based registries on redeemed prescriptions and cancer occurrence respectively, to investigate the effects of exposure to different components and preparations of hormone therapy on breast cancer risk according to hormone receptor status and other tumor characteristics.

Materials and Methods

Study population

The setting of this study has been described in detail elsewhere (18). Briefly, the study population consisted of all women born in Norway 1925–1959, alive and not emigrated as of January 1, 2004 (ages 45–79 years). This population was linked with the Norwegian Prescription Database (NorPD), which includes information on all redeemed prescriptions since 2004, and the incidence database at the Cancer Registry of Norway, which includes all cancer cases since 1953. Information on number of births, age at first birth, vital status, and emigration was obtained from the Norwegian population registry and Statistics Norway. Linkage between the registries was done using the unique personal identification number given to all Norwegian citizens at birth or immigration. The study was approved by the regional ethics committee in the South East region of Norway, and the Norwegian Data Protection Authority.

Among the 895,281 initially identified women, we excluded women born outside of Norway (n = 94,333), women with a cancer diagnosis before study start (n = 52,074), or a breast cancer diagnosis in the first 3 months of the study period (January–March 2004; n = 531). We also excluded women with prescriptions of sex hormones other than ET, tibolone, or EPT during the study period (n = 33,299) and women who only redeemed one prescription after July 1, 2004 (n = 28,430). This left 686,614 women for analyses. The women were followed for incident breast cancer and use of hormone therapy until December 31, 2008.

Identification of breast cancer cases

Cancer reporting is mandatory by law in Norway, and the Cancer Registry of Norway incidence database is 99% complete for solid tumors, including breast cancer (37). The registry records age at diagnosis, histologic grade, tumor size, lymph node involvement, and histologic subtype. Tumor size and lymph node involvement are classified according to the pTNM classification system and histologic grade is determined by the Nottingham criteria (38, 39). In the study period 2004–2008, information on ER and PR receptor status was available only for women who attended the national mammography screening program, which includes screen-detected and interval cancer cases diagnosed in women in the age range 50–69 years (plus 2-year follow-up for interval cancers). We used the cut-off points for ER and PR positivity defined by each laboratory, for most laboratories this was 10%. Among 7,910 incident invasive breast cancer cases in this study, ER status was available for 4,228 (53.5%) cases, and PR status was available for 4,208 (53.2%) cases.

Postmenopausal hormone use

Data on prescriptions redeemed for sex hormones [Anatomical Therapeutic Chemical (ATC) group G03] in the period 2004–2008 were retrieved from the Norwegian Prescription Database (www.norpd.no). Use of hormone therapy was defined as prescriptions for ET or EPT (ATC codes G03C and G03F, respectively). Hormone therapy in Norway includes the estrogen compounds estradiol and estriol, other estrogens (tibolone, with estrogenic, progestogenic, and weak androgenic activity), and combined regimens of estrogen–progestin containing estradiol and norethisterone acetate (NETA; Activelle, Kliogest, Trisekvens, Novofem). Use of progestin types other than NETA is almost nonexistent in Norway (40). Vaginal preparations of estradiol (Vagifem) and estriol (Ovesterin) are used for treatment of vaginal atrophy symptoms and contain lower amounts of estrogens compared with the oral formulations. Vaginal preparations are available without prescription in Norway, which means that the real use has been underestimated in our study.

All women redeeming a prescription for hormone therapy were considered as hormone therapy users. Duration of hormone therapy use was estimated for each hormone type as number of total treatment days, calculated from the package size, multiplied by the number of packages prescribed, and using the recommended dosing intervals. The women were included in the various type of hormone therapy preparation categories based on the specific product dispensed.

Follow-up

Person-years at risk for all women in the study population were calculated from start of the study period until censoring or end of follow-up. Women were censored at death, emigration, breast cancer diagnosis, other cancer diagnosis, or end of follow-up (December 31, 2008), whichever date occurred first. Women contributed person-years at risk as current users according to the accumulated duration of treatment for the type of hormone therapy dispensed. Nonusers contributed person-years at risk from January 1, 2004, until the date of the first redeemed prescription, if any, or end of the follow up. Breast cancer cases occurring during the first 3 months after start of use (current user) or after cessation of use (past user) were allocated to the former hormone therapy status category. These women contributed person-years to the former hormone therapy status from the estimated duration of use until the date of breast cancer diagnosis.

Hormone therapy use was categorized according to type of oral hormone therapy components and products and includes nonuser, past-user, estradiol, estriol, tibolone, Activelle, Kliogest, Trisekvens, and other types (which included users of vaginal and transdermal formulations, women who redeemed concurrent prescriptions of different components or preparations, and users of a number of other less representative hormone therapy preparations). Activelle, Kliogest, and Trisekvens are oral estradiol–NETA preparations of EPT. Activelle (1 mg estradiol and 0.5 mg NETA per day) and Kliogest (2 mg estradiol and 1 mg NETA per day) are continuous regimens. Trisekvens is a sequential EPT formulation with two tablet strengths of 2 or 4 mg estradiol for 22 days followed by 1 mg estradiol for 6 days, in addition to 1 mg NETA for 10 days in a 4 weeks cycle.

Statistical analysis

Incidence rate ratios (RR) with 95% confidence intervals (95% CI) of breast cancer associated with use of hormone therapy were estimated by Poisson regression. All regression models were adjusted for age in 5-year groups (45–49, 50–54, 55–59, 60–64, 65–69, 70–74, 75–79 years), number of births (nulliparous, 1, 2, 3, ≥4), age at first live birth (nulliparous, <25, 25–29, ≥30), and exposure time measured as person-years at risk (time offset in the Poisson regression model). We assessed whether risk differed by tumor characteristics: histologic grade (I, II, and III), tumor size (≤1 cm, >1 and ≥2 cm, and > 2 cm), lymph node involvement (negative and positive), histology type (invasive ductal carcinoma and invasive lobular carcinoma), and for the subset with available data, ER status (negative and positive), PR status (negative and positive), and ER/PR status (ER+ PR+, ER+ PR−, and ER− PR−). Nonusers of hormone therapy were the reference group. All tests were two-sided with a 5% significance level. Statistical analyses were performed using SAS 9.2 (SAS Institute Inc.).

As hormone therapy users are more likely to undergo mammography, we assessed the effect of mammographic screening by calculating the risk estimates for hormone therapy use of the different components and formulations among screen and nonscreen detected cancers.

Results

The analysis database included 686,614 women that accumulated 3.3 million women-years, corresponding to an average follow-up of 4.8 years. A total of 178,383 women (26%) had redeemed hormone therapy prescriptions during the study period. The number of incident invasive breast cancer cases was 7,910.

The average duration of use of the formulations studied were 3.2 years for estradiol, 2.8 years for estriol, 2.7 years for tibolone, 2.6 years for Kliogest, 2.7 years for Activelle, and 2.3 years for Trisekvens. Oral estriol users were older at breast cancer diagnosis compared with nonusers, with 43.1% of estriol users 75 years or older, whereas users of Trisekvens were younger (Table 1). Compared with nonusers, the proportion of lobular tumors was higher among users of Activelle, whereas users of Kliogest and Activelle had a higher proportion of histologic grade I tumors and a lower proportion of grade III tumors. Tumors smaller than 1 cm, lymph node negative tumors, and ER+, PR+, and ER+/PR+ tumors were more frequent among users of estradiol–NETA preparations of Activelle, whereas users of oral tibolone had a higher proportion of PR+ and ER+/PR+ tumors.

View this table:
  • View inline
  • View popup
Table 1.

Breast cancer characteristics by type of oral hormone therapy use assessed at the end of the follow-up period, 2004–2008 (N = 7,910)

Figures 1 and 2 show the associations between hormone therapy use and tumors with varying grade of differentiation, lymph node involvement, and tumor size. Considering nonusers of hormone therapy as the reference, use of oral estradiol, tibolone, Kliogest, Activelle, and Trisekvens was strongly associated with histologic grade I tumors, lymph node involvement negative tumors, and tumors sized ≤ 1 cm and 1 to 2 cm, but the association was weaker and less consistent with tumors with higher histologic grade, positive lymph node involvement (Fig. 1), and tumors >2 cm (Fig. 2). Use of the estradiol–NETA preparations Kliogest, Activelle, and Trisekvens was associated with a nearly 3-fold elevated risk of grade II tumors, and a 2- to 3-fold elevated risk of lymph node–positive tumors, as well as tumors >2 cm. For users of Kliogest there was no substantial difference in the risk estimates between the node positive or negative tumors, and between tumors of different sizes.

Figure 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1.

Relative risk of invasive breast cancer by current use of different types of oral hormone therapy, histologic grade, and lymph node involvement. Adjusted for age (5-year), number of births, age at first birth and time (offset). *Others include vaginal/transdermal formulations, users of concurrent products, and users of other products not listed.

Figure 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 2.

Relative risk of invasive breast cancer by current use of different types of oral hormone therapy and tumor size. Adjusted for age (5-year), number of births, age at first birth and time (offset). *Others include vaginal/transdermal formulations, users of concurrent products, and users of other products not listed.

Use of oral formulations of estradiol was associated with a modestly increased risk of invasive ductal tumors and a 2-fold increased risk of lobular cancers (Fig. 3), whereas users of tibolone had an almost double risk of both ductal and lobular tumors. Use of estradiol–NETA preparations of Kliogest, Activelle, and Trisekvens was associated with a 2.5- to 3-fold increased risk of invasive ductal cancers and a 3.7- to 4.6-fold increased risk of invasive lobular tumors (Fig. 3).

Figure 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 3.

Relative risk of invasive breast cancer by current use of different types of oral hormone therapy and histology type. Adjusted for age (5-year), number of births, age at first birth and time (offset). *Others include vaginal/transdermal formulations, users of concurrent products, and users of other products not listed.

Use of oral estradiol, tibolone, Kliogest, Activelle, and Trisekvens was more strongly associated with hormone receptor positive than receptor negative tumors (Fig. 4). However, use of Kliogest and Activelle was associated with a 2-fold elevated risk of PR− tumors compared with nonusers, and use of the three estradiol–NETA preparations (Kliogest, Activelle, and Trisekvens) was associated with an increased risk of ER+/PR− tumors compared with nonusers.

Figure 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 4.

Relative risk of invasive breast cancer by current use of different types of oral hormone therapy and hormone receptor status. Adjusted for age (5-year), number of births, age at first birth and time (offset). *Others include vaginal/transdermal formulations, users of concurrent products, and users of other products not listed.

The sensitivity analyses showed remarkably similar results as an overall, by histologic grade, by lymph node involvement, and by tumor size regardless of whether the cancer was detected at or outside of the national breast cancer screening program (Supplementary Tables S1–S4).

Discussion

In this large population-based cohort study, with exposure to hormone therapy based on a national registry on redeemed hormone therapy prescriptions in Norway, we found that, in general, use of the hormone therapy preparations studied was strongly associated with tumors with good prognostic characteristics (histologic grade I, no lymph node involvement, tumor size ≤ 2 cm, and hormone receptor–positive tumors) and invasive lobular cancers. However, we found that use of estradiol–NETA preparations (Kliogest, Activelle, and Trisekvens) was also associated with a 2- to 3-fold elevated risks of tumors with histologic grade II, lymph node involvement, and size >2 cm, and that Kliogest in particular was equally strongly associated with cancers with negative and positive lymph node involvement, and small and large size tumors.

Tumor characteristics

A number of studies have reported that hormone therapy use is predominantly associated with tumors with good prognosis characteristics (2, 22). A previous study found that hormone therapy use was more strongly associated with histologic grade I or II compared with grade III and also with a better prognosis score based on histologic grade, lymph node involvement, and tumor size (22). However, the increased risk of grade II, positive lymph node, and large size tumors found in our study with use of tibolone and estradiol–NETA is in agreement with a study that found an increased risk of tumors sized 2 to 5 cm among ET and EPT users (17). This finding supports the hypothesis that specific ET and EPT components carry an increased risk of breast cancer not limited to localized good prognosis tumors.

Histologic subtype

We found that use of oral estradiol, tibolone, and estradiol–NETA preparations (Kliogest, Activelle, and Trisekvens) were associated with both invasive ductal and invasive lobular carcinomas, with a stronger association for invasive lobular compared with invasive ductal cancers. Our results of an increased risk for invasive ductal carcinoma with current use of ET and EPT has previously been reported (26, 29, 31, 32, 36, 41–44), although other published studies showed no association (21, 27, 28, 34, 35). A greater risk of lobular cancer or mixed ductal–lobular cancer compared with ductal cancer are in agreement with most published studies (21, 23, 26–28, 30–32, 34–36, 43, 44), but not with others (15). Invasive lobular cancers have been associated with a better short- and long-term survival and a better stage-matched prognosis than women with invasive ductal cancer (45, 46), although the data are not completely consistent (47).

Hormone receptors of breast cancers

Previous studies have shown a stronger association of hormone therapy use with hormone receptor–positive breast tumors (2, 17, 23, 24, 29). Consistently, we found that use of estradiol, tibolone, and estradiol–NETA preparations of Kliogest, Activelle, and Trisekvens was most strongly associated with ER+ and PR+ tumors. The risk was also elevated for ER+/PR− tumors whereas no association was found for ER−/PR− tumors. This is consistent with other studies (17, 23), although lack of statistical power has to be considered for ER−/PR− tumors. There were too few ER−PR+ tumors (1.1% of all breast malignancies), to provide any reliable estimates for this subgroup.

Tibolone

To our knowledge, this is the first study to analyze the association of tibolone with tumor characteristics. Tibolone is a tissue-specific regulator claimed to be less risky for the breast than natural estrogen therapies (48, 49). However, we found tibolone to be more strongly associated with the various tumor subgroups studied than natural estrogen components of estradiol. Previous studies have shown an increased breast cancer risk in tibolone users compared with ET users (10, 19). Similarly to EPT users, we found that use of tibolone was associated with an overrepresentation of tumor subgroups conferring a favorable prognosis, but also with an elevated risk of more advanced breast cancers with less beneficial characteristics, although the intensity of the associations was of a lesser magnitude.

Estradiol–NETA preparations

Several studies have suggested that the higher risks found in European compared with U.S. studies might be partially explained by the greater use of estradiol–NETA in Europe as opposed to the more common medroxyprogesterone in the United States (50). However, few studies have been able to directly compare these medications, as most populations use either one or the other. Those that have attempted have not yielded consistent results (10, 36, 51). We found a strong and consistently increased risk associated with use of estradiol–NETA preparations. The lower dose preparation Activelle (1 mg estradiol and 0.5 mg NETA daily) conferred similarly increased risks for good prognosis characteristics to the highest NETA dose preparation Kliogest (2 mg estradiol and 1 mg NETA daily). However, we found a significantly higher risk for lymph node positive and large tumors in users of Kliogest compared with Activelle. The high risk of poor prognosis characteristics associated with the high-dose preparation Kliogest is of particular concern. Prescribing the lowest biologically effective dose of progestin might be expected to minimize the risk of breast cancer. Although Kliogest was withdrawn from the Norwegian market in 2010, it is still available elsewhere. Activelle is currently being used in Norway and in a number of other countries. It should be noted that our statistical power to test some of the differences between formulations was limited and should be considered carefully.

Breast cancer detection by mammography

Women who go to regular screening mammography are more likely to be diagnosed with an early-stage cancer, but may also be more likely to use hormone therapy (52). We did not have individual-level information on screening history of women in the study population, but we had information on whether breast cancers were detected at mammography screening or outside the screening program. Interestingly, the sensitivity analyses comparing screen and nonscreen detected cancers showed remarkably similar results regardless of whether the cancer was detected within or outside the screening program.

Strengths and limitations

A major strength of this study is that the data on hormone therapy exposure and breast cancer occurrence was obtained from linkage of nationwide population-based registries. All redeemed prescriptions of hormone therapy and all detected breast cancers in Norwegian women ages 45 to 79 years by 2004 are included. The information on components, preparations, and duration of use of each preparation adds to the strength, as opposed to self-reported hormone therapy use. The Cancer Registry was established in 1952, which means that we were able to exclude all women with earlier or prevalent cancer by 2004. The study included nearly 700,000 women, which provided sufficient statistical power to analyze various tumor subgroups and hormone therapy exposures.

However, the study has several limitations. First, we had no information on hormone therapy use before study start. A number of current users may have been users prior to 2004, so total durations of use may be higher and the risk could be overestimated. However, a number of our presumed never users may have used hormones prior to the study start, which could underestimate the risk. Our study was also limited by the number of women with missing information for some subtype analyses. In particular, ER and PR status were not widely collected for all cases during the study period (available for 53% of breast cancer cases). We also lacked information on possible confounding factors such as age at menopause, income, body mass index, physical activity, or family history of breast cancer. Norwegian data from the 1980s showed that hormone therapy users had higher education and income, were leaner, but differed minimally by physical activity compared with nonusers (53). Nonetheless, we think that neither factor is a strong enough risk factor to have accounted for the observed effects.

Conclusion

Our study suggests that current hormone therapy users of oral estradiol, tibolone, and estradiol–NETA preparations had an increased risk of good prognosis tumors. However, users of estradiol–NETA preparations were at a 2- to 3-fold elevated risk of grade II tumors, breast cancers with lymph node involvement, and tumors >2 cm. Thus, the hormone therapy preparations most commonly used in the Nordic countries were associated with breast cancers with both good and less good prognostic characteristics.

Disclosure of Potential Conflicts of Interest

The authors declare that they have no conflicts of interest.

Authors' Contributions

Conception and design: M. Román, E. Weiderpass, S. Vangen, S. Sakshaug, S. Hofvind, G. Ursin

Development of methodology: M. Román, E. Weiderpass, S. Vangen, S. Hofvind, G. Ursin

Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): M. Román, S. Graff-Iversen, E. Weiderpass, S. Vangen, S. Hofvind, G. Ursin

Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis): M. Román, E. Weiderpass, S. Sakshaug, S. Hofvind, G. Ursin

Writing, review, and/or revision of the manuscript: M. Román, S. Graff-Iversen, E. Weiderpass, S. Vangen, S. Sakshaug, S. Hofvind, G. Ursin

Administrative, technical, or material support (i.e., reporting or organizing data, constructing databases): M. Román, E. Weiderpass, S. Hofvind, G. Ursin

Study supervision: M. Román, E. Weiderpass, S. Vangen, S. Hofvind

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Footnotes

  • Note: Supplementary data for this article are available at Cancer Epidemiology, Biomarkers & Prevention Online (http://cebp.aacrjournals.org/).

  • Received March 24, 2016.
  • Revision received July 6, 2016.
  • Accepted July 16, 2016.
  • ©2016 American Association for Cancer Research.

References

  1. 1.↵
    1. Colditz GA,
    2. Hankinson SE,
    3. Hunter DJ,
    4. Willett WC,
    5. Manson JE,
    6. Stampfer MJ,
    7. et al.
    The use of estrogens and progestins and the risk of breast cancer in postmenopausal women. N Engl J Med 1995;332:1589–93.
    OpenUrlCrossRefPubMed
  2. 2.↵
    Breast cancer and hormone replacement therapy: Collaborative reanalysis of data from 51 epidemiological studies of 52,705 women with breast cancer and 108,411 women without breast cancer. Collaborative group on hormonal factors in breast cancer. Lancet 1997;350:1047–59.
    OpenUrlCrossRefPubMed
  3. 3.↵
    1. Persson I,
    2. Weiderpass E,
    3. Bergkvist L,
    4. Bergstrom R,
    5. Schairer C
    . Risks of breast and endometrial cancer after estrogen and estrogen-progestin replacement. Cancer Causes Control 1999;10:253–60.
    OpenUrlCrossRefPubMed
  4. 4.↵
    1. Magnusson C,
    2. Baron JA,
    3. Correia N,
    4. Bergstrom R,
    5. Adami HO,
    6. Persson I
    . Breast-cancer risk following long-term oestrogen- and oestrogen-progestin-replacement therapy. Int J Cancer 1999;81:339–44.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Colditz GA,
    2. Rosner B
    . Cumulative risk of breast cancer to age 70 years according to risk factor status: data from the nurses' health study. Am J Epidemiol 2000;152:950–64.
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Ross RK,
    2. Paganini-Hill A,
    3. Wan PC,
    4. Pike MC
    . Effect of hormone replacement therapy on breast cancer risk: estrogen versus estrogen plus progestin. J Natl Cancer Inst 2000;92:328–32.
    OpenUrlAbstract/FREE Full Text
  7. 7.↵
    1. Schairer C,
    2. Lubin J,
    3. Troisi R,
    4. Sturgeon S,
    5. Brinton L,
    6. Hoover R
    . Menopausal estrogen and estrogen-progestin replacement therapy and breast cancer risk. JAMA 2000;283:485–91.
    OpenUrlCrossRefPubMed
  8. 8.↵
    1. Rossouw JE,
    2. Anderson GL,
    3. Prentice RL,
    4. LaCroix AZ,
    5. Kooperberg C,
    6. Stefanick ML,
    7. et al.
    Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results from the women's health initiative randomized controlled trial. JAMA 2002;288:321–33.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Beral V,
    2. Banks E,
    3. Reeves G
    . Evidence from randomised trials on the long-term effects of hormone replacement therapy. Lancet 2002;360:942–4.
    OpenUrlCrossRefPubMed
  10. 10.↵
    1. Beral V
    . Breast cancer and hormone-replacement therapy in the million women study. Lancet 2003;362:419–27.
    OpenUrlCrossRefPubMed
  11. 11.↵
    1. Chlebowski RT,
    2. Hendrix SL,
    3. Langer RD,
    4. Stefanick ML,
    5. Gass M,
    6. Lane D,
    7. et al.
    Influence of estrogen plus progestin on breast cancer and mammography in healthy postmenopausal women: the women's health initiative randomized trial. JAMA 2003;289:3243–53.
    OpenUrlCrossRefPubMed
  12. 12.↵
    1. Olsson HL,
    2. Ingvar C,
    3. Bladstrom A
    . Hormone replacement therapy containing progestins and given continuously increases breast carcinoma risk in Sweden. Cancer 2003;97:1387–92.
    OpenUrlCrossRefPubMed
  13. 13.↵
    1. Colditz GA
    . Estrogen, estrogen plus progestin therapy, and risk of breast cancer. Clin Cancer Res 2005;11:909S–17S.
    OpenUrlPubMed
  14. 14.↵
    International Agency for Research on Cancer. Pharmaceuticals. Volume 100 A. A review of human carcinogens. IARC Monogr Eval Carcinog Risks Hum 2012;100:1–401.
    OpenUrlPubMed
  15. 15.↵
    1. Anderson GL,
    2. Limacher M,
    3. Assaf AR,
    4. Bassford T,
    5. Beresford SA,
    6. Black H,
    7. et al.
    Effects of conjugated equine estrogen in postmenopausal women with hysterectomy: the women's health initiative randomized controlled trial. JAMA 2004;291:1701–12.
    OpenUrlCrossRefPubMed
  16. 16.↵
    1. Grosse Y,
    2. Baan R,
    3. Straif K,
    4. Secretan B,
    5. El Ghissassi F,
    6. Bouvard V,
    7. et al.
    A review of human carcinogens-part a: pharmaceuticals. Lancet Oncol 2009;10:13–4.
    OpenUrlCrossRefPubMed
  17. 17.↵
    1. Saxena T,
    2. Lee E,
    3. Henderson KD,
    4. Clarke CA,
    5. West D,
    6. Marshall SF,
    7. et al.
    Menopausal hormone therapy and subsequent risk of specific invasive breast cancer subtypes in the California teachers study. Cancer Epidemiol Biomarkers Prev 2010;19:2366–78.
    OpenUrlAbstract/FREE Full Text
  18. 18.↵
    1. Roman M,
    2. Sakshaug S,
    3. Graff-Iversen S,
    4. Vangen S,
    5. Weiderpass E,
    6. Ursin G,
    7. et al.
    Postmenopausal hormone therapy and the risk of breast cancer in Norway. Int J Cancer 2016;138:584–93.
    OpenUrlPubMed
  19. 19.↵
    1. Stahlberg C,
    2. Pedersen AT,
    3. Lynge E,
    4. Andersen ZJ,
    5. Keiding N,
    6. Hundrup YA,
    7. et al.
    Increased risk of breast cancer following different regimens of hormone replacement therapy frequently used in Europe. Int J Cancer 2004;109:721–7.
    OpenUrlCrossRefPubMed
  20. 20.↵
    1. Formoso G,
    2. Perrone E,
    3. Maltoni S,
    4. Balduzzi S,
    5. D'Amico R,
    6. Bassi C,
    7. et al.
    Short and long term effects of tibolone in postmenopausal women. Cochrane Database Syst Rev 2012;2:CD008536.
    OpenUrlPubMed
  21. 21.↵
    1. Li CI,
    2. Weiss NS,
    3. Stanford JL,
    4. Daling JR
    . Hormone replacement therapy in relation to risk of lobular and ductal breast carcinoma in middle-aged women. Cancer 2000;88:2570–7.
    OpenUrlCrossRefPubMed
  22. 22.↵
    1. Manjer J,
    2. Malina J,
    3. Berglund G,
    4. Bondeson L,
    5. Garne JP,
    6. Janzon L
    . Increased incidence of small and well-differentiated breast tumors in postmenopausal women following hormone-replacement therapy. Int J Cancer 2001;92:919–22.
    OpenUrlCrossRefPubMed
  23. 23.↵
    1. Ursin G,
    2. Tseng CC,
    3. Paganini-Hill A,
    4. Enger S,
    5. Wan PC,
    6. Formenti S,
    7. et al.
    Does menopausal hormone replacement therapy interact with known factors to increase risk of breast cancer? J Clin Oncol 2002;20:699–706.
    OpenUrlAbstract/FREE Full Text
  24. 24.↵
    1. Chen WY,
    2. Hankinson SE,
    3. Schnitt SJ,
    4. Rosner BA,
    5. Holmes MD,
    6. Colditz GA
    . Association of hormone replacement therapy to estrogen and progesterone receptor status in invasive breast carcinoma. Cancer 2004;101:1490–500.
    OpenUrlCrossRefPubMed
  25. 25.↵
    1. Chen WY,
    2. Colditz GA
    . Risk factors and hormone-receptor status: epidemiology, risk-prediction models, and treatment implications for breast cancer. Nat Clin Pract Oncol 2007;4:415–23.
    OpenUrlPubMed
  26. 26.↵
    1. Newcomb PA,
    2. Titus-Ernstoff L,
    3. Egan KM,
    4. Trentham-Dietz A,
    5. Baron JA,
    6. Storer BE,
    7. et al.
    Postmenopausal estrogen and progestin use in relation to breast cancer risk. Cancer Epidemiol Biomarkers Prev 2002;11:593–600.
    OpenUrlAbstract/FREE Full Text
  27. 27.↵
    1. Daling JR,
    2. Malone KE,
    3. Doody DR,
    4. Voigt LF,
    5. Bernstein L,
    6. Coates RJ,
    7. et al.
    Relation of regimens of combined hormone replacement therapy to lobular, ductal, and other histologic types of breast carcinoma. Cancer 2002;95:2455–64.
    OpenUrlCrossRefPubMed
  28. 28.↵
    1. Chen CL,
    2. Weiss NS,
    3. Newcomb P,
    4. Barlow W,
    5. White E
    . Hormone replacement therapy in relation to breast cancer. JAMA 2002;287:734–41.
    OpenUrlCrossRefPubMed
  29. 29.↵
    1. Li CI,
    2. Malone KE,
    3. Porter PL,
    4. Weiss NS,
    5. Tang MT,
    6. Cushing-Haugen KL,
    7. et al.
    Relationship between long durations and different regimens of hormone therapy and risk of breast cancer. JAMA 2003;289:3254–63.
    OpenUrlCrossRefPubMed
  30. 30.↵
    1. Li CI,
    2. Daling JR,
    3. Malone KE,
    4. Bernstein L,
    5. Marchbanks PA,
    6. Liff JM,
    7. et al.
    Relationship between established breast cancer risk factors and risk of seven different histologic types of invasive breast cancer. Cancer Epidemiol Biomarkers Prev 2006;15:946–54.
    OpenUrlAbstract/FREE Full Text
  31. 31.↵
    1. Reeves GK,
    2. Beral V,
    3. Green J,
    4. Gathani T,
    5. Bull D
    . Hormonal therapy for menopause and breast-cancer risk by histological type: a cohort study and meta-analysis. Lancet Oncol 2006;7:910–8.
    OpenUrlCrossRefPubMed
  32. 32.↵
    1. Rosenberg LU,
    2. Magnusson C,
    3. Lindstrom E,
    4. Wedren S,
    5. Hall P,
    6. Dickman PW
    . Menopausal hormone therapy and other breast cancer risk factors in relation to the risk of different histological subtypes of breast cancer: a case-control study. Breast Cancer Res 2006;8:R11.
    OpenUrlCrossRefPubMed
  33. 33.↵
    1. Li CI,
    2. Daling JR,
    3. Haugen KL,
    4. Tang MT,
    5. Porter PL,
    6. Malone KE
    . Use of menopausal hormone therapy and risk of ductal and lobular breast cancer among women 55–74 years of age. Breast Cancer Res Treat 2014;145:481–9.
    OpenUrlPubMed
  34. 34.↵
    1. Newcomer LM,
    2. Newcomb PA,
    3. Potter JD,
    4. Yasui Y,
    5. Trentham-Dietz A,
    6. Storer BE,
    7. et al.
    Postmenopausal hormone therapy and risk of breast cancer by histologic type (United States). Cancer Causes Control 2003;14:225–33.
    OpenUrlCrossRefPubMed
  35. 35.↵
    1. Li CI,
    2. Malone KE,
    3. Porter PL,
    4. Lawton TJ,
    5. Voigt LF,
    6. Cushing-Haugen KL,
    7. et al.
    Relationship between menopausal hormone therapy and risk of ductal, lobular, and ductal-lobular breast carcinomas. Cancer Epidemiol Biomarkers Prev 2008;17:43–50.
    OpenUrlAbstract/FREE Full Text
  36. 36.↵
    1. Flesch-Janys D,
    2. Slanger T,
    3. Mutschelknauss E,
    4. Kropp S,
    5. Obi N,
    6. Vettorazzi E,
    7. et al.
    Risk of different histological types of postmenopausal breast cancer by type and regimen of menopausal hormone therapy. Int J Cancer 2008;123:933–41.
    OpenUrlCrossRefPubMed
  37. 37.↵
    1. Larsen IK,
    2. Smastuen M,
    3. Johannesen TB,
    4. Langmark F,
    5. Parkin DM,
    6. Bray F,
    7. et al.
    Data quality at the cancer registry of Norway: an overview of comparability, completeness, validity, and timeliness. Eur J Cancer 2009;45:1218–31.
    OpenUrlCrossRefPubMed
  38. 38.↵
    1. Wittekind CGF,
    2. Hutter RVP,
    3. Klimpfinger M,
    4. Sobin LH
    . TNM Atlas—illustrated guide to the TNM/pTNM classification of malignant tumours. 5th ed. Heidelberg, Germany: Springer; 2005.
  39. 39.↵
    1. Elston CW,
    2. Ellis IO
    . Pathological prognostic factors in breast cancer. I. The value of histological grade in breast cancer: experience from a large study with long-term follow-up. Histopathology 1991;19:403–10.
    OpenUrlCrossRefPubMed
  40. 40.↵
    1. Hofvind S,
    2. Sakshaug S,
    3. Ursin G,
    4. Graff-Iversen S
    . Breast cancer incidence trends in Norway—explained by hormone therapy or mammographic screening? Int J Cancer 2012;130:2930–8.
    OpenUrlCrossRefPubMed
  41. 41.↵
    1. Lee S,
    2. Kolonel L,
    3. Wilkens L,
    4. Wan P,
    5. Henderson B,
    6. Pike M
    . Postmenopausal hormone therapy and breast cancer risk: the multiethnic cohort. Int J Cancer 2006;118:1285–91.
    OpenUrlCrossRefPubMed
  42. 42.↵
    1. Kotsopoulos J,
    2. Chen WY,
    3. Gates MA,
    4. Tworoger SS,
    5. Hankinson SE,
    6. Rosner BA
    . Risk factors for ductal and lobular breast cancer: results from the nurses' health study. Breast Cancer Res 2010;12:R106.
    OpenUrlCrossRefPubMed
  43. 43.↵
    1. Fournier A,
    2. Fabre A,
    3. Mesrine S,
    4. Boutron-Ruault MC,
    5. Berrino F,
    6. Clavel-Chapelon F
    . Use of different postmenopausal hormone therapies and risk of histology- and hormone receptor-defined invasive breast cancer. J Clin Oncol 2008;26:1260–8.
    OpenUrlAbstract/FREE Full Text
  44. 44.↵
    1. Calle EE,
    2. Feigelson HS,
    3. Hildebrand JS,
    4. Teras LR,
    5. Thun MJ,
    6. Rodriguez C
    . Postmenopausal hormone use and breast cancer associations differ by hormone regimen and histologic subtype. Cancer 2009;115:936–45.
    OpenUrlCrossRefPubMed
  45. 45.↵
    1. Wasif N,
    2. Maggard MA,
    3. Ko CY,
    4. Giuliano AE
    . Invasive lobular vs. ductal breast cancer: a stage-matched comparison of outcomes. Ann Surg Oncol 2010;17:1862–9.
    OpenUrlCrossRefPubMed
  46. 46.↵
    1. Toikkanen S,
    2. Pylkkanen L,
    3. Joensuu H
    . Invasive lobular carcinoma of the breast has better short- and long-term survival than invasive ductal carcinoma. Br J Cancer 1997;76:1234–40.
    OpenUrlCrossRefPubMed
  47. 47.↵
    1. Lehmann U
    . Lobular breast cancer–the most common special subtype or a most special common subtype? Breast Cancer Res 2015;17:99.
    OpenUrlPubMed
  48. 48.↵
    1. Archer DF,
    2. Hendrix S,
    3. Gallagher JC,
    4. Rymer J,
    5. Skouby S,
    6. Ferenczy A,
    7. et al.
    Endometrial effects of tibolone. J Clin Endocrinol Metab 2007;92:911–8.
    OpenUrlCrossRefPubMed
  49. 49.↵
    1. Cummings SR,
    2. Ettinger B,
    3. Delmas PD,
    4. Kenemans P,
    5. Stathopoulos V,
    6. Verweij P,
    7. et al.
    The effects of tibolone in older postmenopausal women. N Engl J Med 2008;359:697–708.
    OpenUrlCrossRefPubMed
  50. 50.↵
    1. Lee SA,
    2. Ross RK,
    3. Pike MC
    . An overview of menopausal oestrogen-progestin hormone therapy and breast cancer risk. Br J Cancer 2005;92:2049–58.
    OpenUrlCrossRefPubMed
  51. 51.↵
    1. Bakken K,
    2. Fournier A,
    3. Lund E,
    4. Waaseth M,
    5. Dumeaux V,
    6. Clavel-Chapelon F,
    7. et al.
    Menopausal hormone therapy and breast cancer risk: impact of different treatments. The European prospective investigation into cancer and nutrition. Int J Cancer 2011;128:144–56.
    OpenUrlCrossRefPubMed
  52. 52.↵
    1. Buist DS,
    2. Walker R,
    3. Bowles EJ,
    4. Carney PA,
    5. Taplin SH,
    6. Onega T,
    7. et al.
    Screening mammography use among current, former, and never hormone therapy users may not explain recent declines in breast cancer incidence. Cancer Epidemiol Biomarkers Prev 2012;21:720–7.
    OpenUrlAbstract/FREE Full Text
  53. 53.↵
    1. Graff-Iversen S,
    2. Hammar N,
    3. Thelle DS,
    4. Tonstad S
    . Hormone therapy and mortality during a 14-year follow-up of 14,324 Norwegian women. J Intern Med 2004;256:437–45.
    OpenUrlPubMed
PreviousNext
Back to top
Cancer Epidemiology Biomarkers & Prevention: 25 (11)
November 2016
Volume 25, Issue 11
  • Table of Contents
  • Table of Contents (PDF)
  • Editorial Board (PDF)

Sign up for alerts

View this article with LENS

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Epidemiology, Biomarkers & Prevention article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Postmenopausal Hormone Therapy and Breast Cancer Prognostic Characteristics: A Linkage between Nationwide Registries
(Your Name) has forwarded a page to you from Cancer Epidemiology, Biomarkers & Prevention
(Your Name) thought you would be interested in this article in Cancer Epidemiology, Biomarkers & Prevention.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Postmenopausal Hormone Therapy and Breast Cancer Prognostic Characteristics: A Linkage between Nationwide Registries
Marta Román, Sidsel Graff-Iversen, Elisabete Weiderpass, Siri Vangen, Solveig Sakshaug, Solveig Hofvind and Giske Ursin
Cancer Epidemiol Biomarkers Prev November 1 2016 (25) (11) 1464-1473; DOI: 10.1158/1055-9965.EPI-16-0240

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Postmenopausal Hormone Therapy and Breast Cancer Prognostic Characteristics: A Linkage between Nationwide Registries
Marta Román, Sidsel Graff-Iversen, Elisabete Weiderpass, Siri Vangen, Solveig Sakshaug, Solveig Hofvind and Giske Ursin
Cancer Epidemiol Biomarkers Prev November 1 2016 (25) (11) 1464-1473; DOI: 10.1158/1055-9965.EPI-16-0240
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Conclusion
    • Disclosure of Potential Conflicts of Interest
    • Authors' Contributions
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Early-Life Risk Factors for Breast Cancer
  • Sugary Drink Consumption and Colorectal Cancer Risk
  • HPV Testing in Self-samples and Urine
Show more Research Articles
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook   Twitter   LinkedIn   YouTube   RSS

Articles

  • Online First
  • Current Issue
  • Past Issues

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Epidemiology, Biomarkers & Prevention

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Epidemiology, Biomarkers & Prevention
eISSN: 1538-7755
ISSN: 1055-9965

Advertisement